Abstract
Tyrosinase (0.2 mg/ml of 0.1 M phosphate buffer solution, pH 6.5) which has cresolase and catecholase activities was irradiated with 60Co gamma-rays. The cresolase activity was measured at varying radiation doses under various atmospheric conditions. Do was found to be 350 Gy in N2-saturated solution. The OH radical has been shown to be the main species involved in radiation-induced enzyme inactivation. However, in this study, OH radical scavengers, t-BuOH and MeOH, had no effect. O2 which acts generally as an enhancer of OH-induced enzyme inactivation also had little effect, but N2O a e(aq)- scavenger, and Cu++ markedly inhibited the inactivation indicating that e(aq)- is the main species involved in inactivating the cresolase activity, reducing Cu++ as the active center.
Original language | English |
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Pages (from-to) | 295-300 |
Number of pages | 6 |
Journal | Biochemistry and Molecular Biology International |
Volume | 34 |
Issue number | 2 |
Publication status | Published - Jan 1 1994 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics