Tyrosinase (0.2 mg/ml of 0.1 M phosphate buffer solution, pH 6.5) which has cresolase and catecholase activities was irradiated with 60Co gamma-rays. The cresolase activity was measured at varying radiation doses under various atmospheric conditions. Do was found to be 350 Gy in N2-saturated solution. The OH radical has been shown to be the main species involved in radiation-induced enzyme inactivation. However, in this study, OH radical scavengers, t-BuOH and MeOH, had no effect. O2 which acts generally as an enhancer of OH-induced enzyme inactivation also had little effect, but N2O a e(aq)- scavenger, and Cu++ markedly inhibited the inactivation indicating that e(aq)- is the main species involved in inactivating the cresolase activity, reducing Cu++ as the active center.
|Number of pages||6|
|Journal||Biochemistry and Molecular Biology International|
|Publication status||Published - Jan 1 1994|
ASJC Scopus subject areas
- Molecular Biology