Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H)

W. K. Born, M. Vollmer, C. Reardon, Eiji Matsuura, D. R. Voelker, P. C. Giclas, R. L. O'Brien

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hybridomas expressing murine γδ T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, β 2-glycoprotein 1 (β2-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. β2-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that γδ T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.

Original languageEnglish
Pages (from-to)374-381
Number of pages8
JournalScandinavian Journal of Immunology
Volume58
Issue number3
DOIs
Publication statusPublished - Sep 1 2003

Fingerprint

beta 2-Glycoprotein I
Cardiolipins
Hybridomas
T-Cell Antigen Receptor
Glycoproteins
Phospholipids
Ovalbumin
Serum
Autoantibodies
Blood Proteins
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H). / Born, W. K.; Vollmer, M.; Reardon, C.; Matsuura, Eiji; Voelker, D. R.; Giclas, P. C.; O'Brien, R. L.

In: Scandinavian Journal of Immunology, Vol. 58, No. 3, 01.09.2003, p. 374-381.

Research output: Contribution to journalArticle

Born, W. K. ; Vollmer, M. ; Reardon, C. ; Matsuura, Eiji ; Voelker, D. R. ; Giclas, P. C. ; O'Brien, R. L. / Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H). In: Scandinavian Journal of Immunology. 2003 ; Vol. 58, No. 3. pp. 374-381.
@article{92a6172b775d4572bee73034de4bc13a,
title = "Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H)",
abstract = "Hybridomas expressing murine γδ T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, β 2-glycoprotein 1 (β2-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. β2-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that γδ T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.",
author = "Born, {W. K.} and M. Vollmer and C. Reardon and Eiji Matsuura and Voelker, {D. R.} and Giclas, {P. C.} and O'Brien, {R. L.}",
year = "2003",
month = "9",
day = "1",
doi = "10.1046/j.1365-3083.2003.01315.x",
language = "English",
volume = "58",
pages = "374--381",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H)

AU - Born, W. K.

AU - Vollmer, M.

AU - Reardon, C.

AU - Matsuura, Eiji

AU - Voelker, D. R.

AU - Giclas, P. C.

AU - O'Brien, R. L.

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Hybridomas expressing murine γδ T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, β 2-glycoprotein 1 (β2-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. β2-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that γδ T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.

AB - Hybridomas expressing murine γδ T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, β 2-glycoprotein 1 (β2-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. β2-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that γδ T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.

UR - http://www.scopus.com/inward/record.url?scp=0042913186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042913186&partnerID=8YFLogxK

U2 - 10.1046/j.1365-3083.2003.01315.x

DO - 10.1046/j.1365-3083.2003.01315.x

M3 - Article

VL - 58

SP - 374

EP - 381

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 3

ER -