Abstract
Hybridomas expressing murine γδ T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, β 2-glycoprotein 1 (β2-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. β2-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that γδ T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.
| Original language | English |
|---|---|
| Pages (from-to) | 374-381 |
| Number of pages | 8 |
| Journal | Scandinavian Journal of Immunology |
| Volume | 58 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 1 2003 |
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ASJC Scopus subject areas
- Immunology
Cite this
Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H). / Born, W. K.; Vollmer, M.; Reardon, C.; Matsuura, Eiji; Voelker, D. R.; Giclas, P. C.; O'Brien, R. L.
In: Scandinavian Journal of Immunology, Vol. 58, No. 3, 01.09.2003, p. 374-381.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hybridomas expressing γδ T-cell receptors respond to cardiolipin and β2-glycoprotein 1 (apolipoprotein H)
AU - Born, W. K.
AU - Vollmer, M.
AU - Reardon, C.
AU - Matsuura, Eiji
AU - Voelker, D. R.
AU - Giclas, P. C.
AU - O'Brien, R. L.
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Hybridomas expressing murine γδ T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, β 2-glycoprotein 1 (β2-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. β2-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that γδ T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.
AB - Hybridomas expressing murine γδ T-cell receptors were found to produce cytokines in response to cardiolipin (CL) and structurally related anionic phospholipids. This response required serum at concentrations related to the amount of CL in cultures. The purified serum factor, β 2-glycoprotein 1 (β2-GP1) (apolipoprotein H), supported the CL response alone, whereas several other serum proteins and ovalbumin did not. β2-GP1 is known to form complexes with anionic phospholipids, particularly CL, which are often recognized by pathological autoantibodies. We speculate that γδ T cells also recognize such complexes and that the hybridoma response reported here reflects this specificity.
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UR - http://www.scopus.com/inward/citedby.url?scp=0042913186&partnerID=8YFLogxK
U2 - 10.1046/j.1365-3083.2003.01315.x
DO - 10.1046/j.1365-3083.2003.01315.x
M3 - Article
VL - 58
SP - 374
EP - 381
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
SN - 0300-9475
IS - 3
ER -