Hybridization quality in cervical cementum and superficial dentin using current adhesives

Yang Yuan, Yasushi Shimada, Shizuko Ichinose, Junji Tagami

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Objectives: The aim of this research was to determine the hybridization quality of adhesives to gingival cementum and close superficial dentin using both total-etch and self-etch, one-step and two-step adhesives in vitro. Methods: Five adhesive systems were used and evaluated in this study; three kinds of two-step adhesives (total-etch-Single Bond and self-etch-Clearfil SE Bond and Clearfil Protect Bond) and two one-step adhesives (Clearfil S3 Bond, G Bond). Fifteen extracted intact human third molars were used in this study. A diagonal cut which was approximately 45° to the long axis of the roots, with the initiating point located 2 mm below the buccal enamel-cementum junction and ascending towards the pulp chamber was prepared on each tooth. Flat cervical cementum and dentin surfaces were ground with wet 600-grit silicon carbide paper, and bonded with one of the adhesives and finished by applying a flowable resin composite. After 24 h storage at 37 °C in water, the bonded assemblies were sectioned into approximately 1 mm thick slabs. Two central slabs from each tooth were chosen. One slab was totally demineralized in 0.5 M EDTA and the other was not demineralized and immersed into 50% (w/v) solution of ammoniacal silver nitrate for 24 h, and successively exposed to photodeveloping solution for 8 h. The specimens were then processed for TEM observation. Both the stained demineralized silver unchallenged and unstained non-demineralized silver challenged resin-cervical cementum/proximal superficial dentin interface were observed and evaluated under a transmission electron microscope. Results: The nanoleakage pathway and extent vary among the different adhesives used and also between the resin-cementum interface and resin-dentin interface. Two-step self-etch adhesives showed better hybridization quality both in cementum and proximal superficial dentin as compared to those of two-step total-etch adhesive and one-step self-etch adhesives. Significance: Two-step self-etch adhesives may provide a better sealing in cervical cementum and the proximal superficial dentin region.

Original languageEnglish
Pages (from-to)584-593
Number of pages10
JournalDental Materials
Volume24
Issue number5
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Keywords

  • Gingival cementum
  • Hybrid layer
  • Nanoleakage
  • Superficial dentin
  • TEM

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

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