Hybrid sterility with meiotic metaphase arrest in intersubspecific mouse crosses

Risako Nishino, Sabrina Petri, Mary Ann Handel, Tetsuo Kunieda, Yasuhiro Fujiwara

Research output: Contribution to journalArticle

Abstract

Although organisms belonging to different species and subspecies sometimes produce fertile offspring, a hallmark of the speciation process is reproductive isolation, characterized by hybrid sterility (HS) due to failure in gametogenesis. In mammals, HS is usually exhibited by males, the heterogametic sex. The phenotypic manifestations of HS are complex. The most frequently observed are abnormalities in both autosomal and sex chromosome interactions that are linked to meiotic prophase arrest or postmeiotic spermiogenesis aberrations and lead to defective or absent gametes. The aim of this study was to determine the HS phenotypes in intersubspecific F1 mice produced by matings between Mus musculus molossinus-derived strains and diverse Mus musculus domesticus-inbred laboratory mouse strains. Most of these crosses produced fertile F1 offspring. However, when female BALB/cJ (domesticus) mice were mated to male JF1/MsJ (molossinus) mice, the (BALBdomxJF1mol)F1 males were sterile, whereas the (JF1molxBALBdom)F1 males produced by the reciprocal crossings were fertile; thus the sterility phenotype was asymmetric. The sterile (BALBdomxJF1mol) F1 males exhibited a high rate of meiotic metaphase arrest with misaligned chromosomes, probably related to a high frequency of XY dissociation. Intriguingly, in the sterile (BALBdomxJF1mol)F1 males we observed aberrant allelespecific expression of several meiotic genes, that play critical roles in important meiotic events including chromosome pairing. Together, these observations of an asymmetrical HS phenotype in intersubspecific F1 males, probably owing to meiotic defects in the meiotic behavior of the XY chromosomes pair and possibly also transcriptional misregulation of meiotic genes, provide new models and directions for understanding speciation mechanisms in mammals.

Original languageEnglish
Pages (from-to)183-193
Number of pages11
JournalJournal of Heredity
Volume110
Issue number2
DOIs
Publication statusPublished - Mar 5 2019

Fingerprint

Metaphase
Infertility
Phenotype
Mammals
Chromosomes
Reproductive Isolation
Gametogenesis
Chromosome Pairing
Prophase
Inbred Strains Mice
Sex Chromosomes
Spermatogenesis
Germ Cells
Genes

Keywords

  • hybrid sterility
  • meiosis
  • metaphase
  • Spo11

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Hybrid sterility with meiotic metaphase arrest in intersubspecific mouse crosses. / Nishino, Risako; Petri, Sabrina; Handel, Mary Ann; Kunieda, Tetsuo; Fujiwara, Yasuhiro.

In: Journal of Heredity, Vol. 110, No. 2, 05.03.2019, p. 183-193.

Research output: Contribution to journalArticle

Nishino, Risako ; Petri, Sabrina ; Handel, Mary Ann ; Kunieda, Tetsuo ; Fujiwara, Yasuhiro. / Hybrid sterility with meiotic metaphase arrest in intersubspecific mouse crosses. In: Journal of Heredity. 2019 ; Vol. 110, No. 2. pp. 183-193.
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