Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush

Toshiki Nishimura, Fuyuko Tamura, Sawako Kobayashi, Yasushi Tanimoto, Fumio Hayashi, Yuki Sudo, Yasuhiko Iwasaki, Kenichi Morigaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Substrate-supported planar lipid bilayers (SPBs) are being utilized as a versatile model system of the biological membrane. However, the proximity between the solid support and membrane limits utility of SPBs for the functional analyses of membrane proteins. Here, we present a model membrane that can enlarge the distance between the substrate surface and the membrane by combining a stable scaffold of polymerized lipid bilayer with a hydrophilic polymer brush. A micropatterned SPB was generated by the lithographic polymerization of diacetylene lipids and subsequent incorporation of natural (fluid) lipid bilayers. Hydrophilic polymer brush of poly-2-methacryloyloxyethyl phosphorylcholine (poly(MPC)) was formed on the surface of polymeric bilayer by the in situ atom transfer radical polymerization (ATRP) in aqueous solution, in the presence of embedded fluid lipid bilayers. A model membrane protein (Haloquadratum walsbyi bacteriorhodopsin: HwBR) could be reconstituted into the polymer brush-supported bilayers with significantly reduced immobile molecules. Furthermore, the polymer brush terminals could be functionalized by successively polymerizing MPC and 2-aminoethyl methacrylate (AMA). The reactive amine moiety of poly(AMA) enables to conjugate a wide range of biological molecules and surfaces to the membrane. The combination of micropatterned bilayer and polymer brush mimics the two- and three-dimensional structures of the biological membrane, providing a platform to assay membrane proteins in a truly biomimetic environment.

Original languageEnglish
Pages (from-to)5752-5759
Number of pages8
JournalLangmuir
Volume33
Issue number23
DOIs
Publication statusPublished - Jun 13 2017

Fingerprint

Lipid bilayers
brushes
Brushes
lipids
Polymers
membranes
Membranes
polymers
Biological membranes
Membrane Proteins
Proteins
proteins
Bacteriorhodopsins
Molecules
Fluids
Atom transfer radical polymerization
Biomimetics
Substrates
polymerization
Scaffolds

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Nishimura, T., Tamura, F., Kobayashi, S., Tanimoto, Y., Hayashi, F., Sudo, Y., ... Morigaki, K. (2017). Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush. Langmuir, 33(23), 5752-5759. https://doi.org/10.1021/acs.langmuir.7b00463

Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush. / Nishimura, Toshiki; Tamura, Fuyuko; Kobayashi, Sawako; Tanimoto, Yasushi; Hayashi, Fumio; Sudo, Yuki; Iwasaki, Yasuhiko; Morigaki, Kenichi.

In: Langmuir, Vol. 33, No. 23, 13.06.2017, p. 5752-5759.

Research output: Contribution to journalArticle

Nishimura, T, Tamura, F, Kobayashi, S, Tanimoto, Y, Hayashi, F, Sudo, Y, Iwasaki, Y & Morigaki, K 2017, 'Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush', Langmuir, vol. 33, no. 23, pp. 5752-5759. https://doi.org/10.1021/acs.langmuir.7b00463
Nishimura, Toshiki ; Tamura, Fuyuko ; Kobayashi, Sawako ; Tanimoto, Yasushi ; Hayashi, Fumio ; Sudo, Yuki ; Iwasaki, Yasuhiko ; Morigaki, Kenichi. / Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush. In: Langmuir. 2017 ; Vol. 33, No. 23. pp. 5752-5759.
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