Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex

Hiroshi Ueno, Shunsuke Suemitsu, Shinji Murakami, Naoya Kitamura, Kenta Wani, Yousuke Matsumoto, Shozo Aoki, Motoi Okamoto, Takeshi Ishihara

Research output: Contribution to journalArticle

Abstract

In the central nervous system (CNS), extracellular matrix (ECM) molecules comprise more than 20% of the volume and are involved in neuronal plasticity, synaptic transmission, and differentiation. Perineuronal nets (PNNs) are ECM molecules that highly accumulate around the soma of neurons. The components of the ECM in the CNS include proteins, proteoglycans, and glycosaminoglycans. Although hyaluronic acid (HA) is considered a constituent element of PNNs, the distribution of HA in the cortex has not been clarified. To elucidate the cortical region-specific distribution of HA, we quantitatively analyzed HA binding protein (HABP)-positive PNNs in the mature mouse cerebral cortex. Our findings revealed that HABP-positive PNNs are present throughout the mouse cortex. The distribution of many HABP-positive PNNs differed from that of Wisteria floribunda agglutinin-positive PNNs. Furthermore, we observed granular-like HABP-positive PNNs in layer 1 of the cortex. These findings indicate that PNNs in the mouse cortex show region-dependent differences in composition. HABP-positive PNNs in layer 1 of the cortex may have different functions such as neuronal differentiation, proliferation, and migration unlike what has been reported for PNNs so far.

Original languageEnglish
Pages (from-to)139-150
Number of pages12
JournalBrain Research
Volume1698
DOIs
Publication statusPublished - Nov 1 2018

Fingerprint

Hyaluronic Acid
Cerebral Cortex
Carrier Proteins
Extracellular Matrix
Central Nervous System
CD44 Antigens
Neuronal Plasticity
Carisoprodol
Proteoglycans
Glycosaminoglycans
Synaptic Transmission
Neurons
Proteins

Keywords

  • Central nervous system
  • Extracellular matrix
  • Glycosaminoglycans
  • Proteoglycans
  • Wisteria floribunda

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex. / Ueno, Hiroshi; Suemitsu, Shunsuke; Murakami, Shinji; Kitamura, Naoya; Wani, Kenta; Matsumoto, Yousuke; Aoki, Shozo; Okamoto, Motoi; Ishihara, Takeshi.

In: Brain Research, Vol. 1698, 01.11.2018, p. 139-150.

Research output: Contribution to journalArticle

Ueno, H, Suemitsu, S, Murakami, S, Kitamura, N, Wani, K, Matsumoto, Y, Aoki, S, Okamoto, M & Ishihara, T 2018, 'Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex', Brain Research, vol. 1698, pp. 139-150. https://doi.org/10.1016/j.brainres.2018.08.011
Ueno, Hiroshi ; Suemitsu, Shunsuke ; Murakami, Shinji ; Kitamura, Naoya ; Wani, Kenta ; Matsumoto, Yousuke ; Aoki, Shozo ; Okamoto, Motoi ; Ishihara, Takeshi. / Hyaluronic acid is present on specific perineuronal nets in the mouse cerebral cortex. In: Brain Research. 2018 ; Vol. 1698. pp. 139-150.
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