Hyaluronan suppresses mechanical stress-induced expression of catabolic enzymes by human chondrocytes via inhibition of IL-1β production and subsequent NF-κB activation

Masatsugu Ozawa, Keiichiro Nishida, Aki Yoshida, Taichi Saito, Ryozo Harada, Takahiro Machida, Toshifumi Ozaki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: To investigate the inhibitory effect of hyaluronan (HA) on mechanical stress- induced expression of a disintegrin and metalloproteinase with thrombospondin type1 motifs (ADAMTS)-4, -5 and matrix metalloproteinase (MMP)-13 by human chondrocytes.Materials and methods: Normal human articular chondrocytes were pre-incubated with or without 1.0 mg/mL HA (2700 kDa) for 12 h at 37 °C in stretch chambers, then they were exposed to uni-axial cyclic tensile strain (CTS, 0.5 Hz, 10 % elongation). The expression of ADAMTS-4, -5, and MMP-13 were analyzed by real-time polymerase chain reaction and Immunocytochemistry. The concentration of IL-1β in the supernatant was measured using enzyme-linked immunosorbent assay (ELISA). The nuclear translocation of runt-related transcription factor 2 (RUNX-2) and nuclear factor-κB (NF-κB) was examined by ELISA and immunocytochemistry, and phosphorylation of NF-κB was examined by western blotting.Results: HA inhibited mRNA expression of ADAMTS-4, -5, and MMP13 after 24 h CTS via inhibition of IL-1β secretion and NF-κB activation. However, HA failed to inhibit CTS-induced RUNX-2 expression and subsequent expression of ADAMTS-5 and MMP-13 1 h after CTS.Conclusions: Our results demonstrated that HA significantly suppressed mechanical stress-induced expression of catabolic proteases by inhibition of the NF-κB–IL-1β pathway, but did not suppress mechanical stress-induced RUNX-2 signaling.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalInflammation Research
Volume64
Issue number3-4
DOIs
Publication statusPublished - Mar 13 2015

Keywords

  • Aggrecanase
  • Chondrocyte
  • Hyaluronan
  • Mechanical stress
  • NF-κB
  • RUNX-2

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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