Hyaluronan stimulates chondrogenic gene expression in human meniscus cells

Takaaki Tanaka, Takayuki Furumatsu, Shinichi Miyazawa, Masataka Fujii, Hiroto Inoue, Yuya Kodama, Toshihumi Ozaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. Materials and Methods: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco’s modified Eagle’s medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2–7 days. Cellular proliferation, migration, and polymerase chain reaction analyses were performed for the inner and outer cells separately. Meniscal samples perforated by a 2 mm diameter punch were maintained for 3 weeks in hyaluronan-supplemented medium and evaluated by histological analyses. Results: Hyaluronan increased the proliferation and migration of both meniscus cell types. Moreover, cellular counts at the surface of both meniscal tissue perforations were increased by hyaluronan treatments. In addition, hyaluronan stimulated α1(II) collagen expression in inner meniscus cells. Accumulation of type II collagen at the perforated surface of both meniscal samples was induced by hyaluronan treatment. Hyaluronan did not induce type I collagen accumulation around the injured site of the meniscus. Conclusion: Hyaluronan stimulated the proliferation and migration of meniscus cells. Our results suggest that hyaluronan may promote the healing potential of meniscus cells in damaged meniscal tissues.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalConnective Tissue Research
DOIs
Publication statusAccepted/In press - Jan 16 2017

Fingerprint

Hyaluronic Acid
Gene expression
Gene Expression
Meniscus
Tissue
Tibial Meniscus
Phenotype
Collagen Type II
Polymerase chain reaction
Eagles
Collagen Type I
Cell Movement
Collagen
Cell Proliferation
Polymerase Chain Reaction

Keywords

  • Chondrocytic gene expression
  • hyaluronan
  • inner meniscus
  • meniscus
  • outer meniscus
  • type II collagen

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Hyaluronan stimulates chondrogenic gene expression in human meniscus cells. / Tanaka, Takaaki; Furumatsu, Takayuki; Miyazawa, Shinichi; Fujii, Masataka; Inoue, Hiroto; Kodama, Yuya; Ozaki, Toshihumi.

In: Connective Tissue Research, 16.01.2017, p. 1-11.

Research output: Contribution to journalArticle

@article{859e8daa1ec84226895bce03cb03b0c4,
title = "Hyaluronan stimulates chondrogenic gene expression in human meniscus cells",
abstract = "Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. Materials and Methods: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco’s modified Eagle’s medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2–7 days. Cellular proliferation, migration, and polymerase chain reaction analyses were performed for the inner and outer cells separately. Meniscal samples perforated by a 2 mm diameter punch were maintained for 3 weeks in hyaluronan-supplemented medium and evaluated by histological analyses. Results: Hyaluronan increased the proliferation and migration of both meniscus cell types. Moreover, cellular counts at the surface of both meniscal tissue perforations were increased by hyaluronan treatments. In addition, hyaluronan stimulated α1(II) collagen expression in inner meniscus cells. Accumulation of type II collagen at the perforated surface of both meniscal samples was induced by hyaluronan treatment. Hyaluronan did not induce type I collagen accumulation around the injured site of the meniscus. Conclusion: Hyaluronan stimulated the proliferation and migration of meniscus cells. Our results suggest that hyaluronan may promote the healing potential of meniscus cells in damaged meniscal tissues.",
keywords = "Chondrocytic gene expression, hyaluronan, inner meniscus, meniscus, outer meniscus, type II collagen",
author = "Takaaki Tanaka and Takayuki Furumatsu and Shinichi Miyazawa and Masataka Fujii and Hiroto Inoue and Yuya Kodama and Toshihumi Ozaki",
year = "2017",
month = "1",
day = "16",
doi = "10.1080/03008207.2016.1264944",
language = "English",
pages = "1--11",
journal = "Connective Tissue Research",
issn = "0300-8207",
publisher = "Informa Healthcare",

}

TY - JOUR

T1 - Hyaluronan stimulates chondrogenic gene expression in human meniscus cells

AU - Tanaka, Takaaki

AU - Furumatsu, Takayuki

AU - Miyazawa, Shinichi

AU - Fujii, Masataka

AU - Inoue, Hiroto

AU - Kodama, Yuya

AU - Ozaki, Toshihumi

PY - 2017/1/16

Y1 - 2017/1/16

N2 - Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. Materials and Methods: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco’s modified Eagle’s medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2–7 days. Cellular proliferation, migration, and polymerase chain reaction analyses were performed for the inner and outer cells separately. Meniscal samples perforated by a 2 mm diameter punch were maintained for 3 weeks in hyaluronan-supplemented medium and evaluated by histological analyses. Results: Hyaluronan increased the proliferation and migration of both meniscus cell types. Moreover, cellular counts at the surface of both meniscal tissue perforations were increased by hyaluronan treatments. In addition, hyaluronan stimulated α1(II) collagen expression in inner meniscus cells. Accumulation of type II collagen at the perforated surface of both meniscal samples was induced by hyaluronan treatment. Hyaluronan did not induce type I collagen accumulation around the injured site of the meniscus. Conclusion: Hyaluronan stimulated the proliferation and migration of meniscus cells. Our results suggest that hyaluronan may promote the healing potential of meniscus cells in damaged meniscal tissues.

AB - Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. Materials and Methods: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco’s modified Eagle’s medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2–7 days. Cellular proliferation, migration, and polymerase chain reaction analyses were performed for the inner and outer cells separately. Meniscal samples perforated by a 2 mm diameter punch were maintained for 3 weeks in hyaluronan-supplemented medium and evaluated by histological analyses. Results: Hyaluronan increased the proliferation and migration of both meniscus cell types. Moreover, cellular counts at the surface of both meniscal tissue perforations were increased by hyaluronan treatments. In addition, hyaluronan stimulated α1(II) collagen expression in inner meniscus cells. Accumulation of type II collagen at the perforated surface of both meniscal samples was induced by hyaluronan treatment. Hyaluronan did not induce type I collagen accumulation around the injured site of the meniscus. Conclusion: Hyaluronan stimulated the proliferation and migration of meniscus cells. Our results suggest that hyaluronan may promote the healing potential of meniscus cells in damaged meniscal tissues.

KW - Chondrocytic gene expression

KW - hyaluronan

KW - inner meniscus

KW - meniscus

KW - outer meniscus

KW - type II collagen

UR - http://www.scopus.com/inward/record.url?scp=85009724003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85009724003&partnerID=8YFLogxK

U2 - 10.1080/03008207.2016.1264944

DO - 10.1080/03008207.2016.1264944

M3 - Article

C2 - 27898233

AN - SCOPUS:85009724003

SP - 1

EP - 11

JO - Connective Tissue Research

JF - Connective Tissue Research

SN - 0300-8207

ER -