Two types of monoclonal antibodies, N4 and N6, were raised against human copper, zinc-superoxide dismutase (Cu, Zn-SOD). An enzyme-linked immunosorbent assay for Cu, Zn-SOD was developed in sandwich method using these monoclonal antibodies. The measurable assay range was 1.0-100 gmg/l and the coefficient of variation within and between series were 7.5% and 8.1%. Sera of 154 healthy control subjects displayed a low level (14 μg/l) of immunoreactive Cu, Zn-SOD with a logarithmic normal distribution. On the other hand, serum Cu, Zn-SOD levels were significantly elevated in patients with various digestive cancers.
- Digestive cancer
- Enzyme-linked immunosorbent assay (ELISA)
- Human copper, zinc-superoxide dismutase (Cu, Zn-SOD)
- Monoclonal antibody
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical