Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer

Shinji Oka, Keiki Ogino, Shinjiro Matsuura, Syunji Yoshimura, Kazunari Yamamoto, Yukinori Okazaki, Tadayoshi Takemoto, Nobuyasu Kato, Taizo Uda

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Abstract

Two types of monoclonal antibodies, N4 and N6, were raised against human copper, zinc-superoxide dismutase (Cu, Zn-SOD). An enzyme-linked immunosorbent assay for Cu, Zn-SOD was developed in sandwich method using these monoclonal antibodies. The measurable assay range was 1.0-100 gmg/l and the coefficient of variation within and between series were 7.5% and 8.1%. Sera of 154 healthy control subjects displayed a low level (14 μg/l) of immunoreactive Cu, Zn-SOD with a logarithmic normal distribution. On the other hand, serum Cu, Zn-SOD levels were significantly elevated in patients with various digestive cancers.

Original languageEnglish
Pages (from-to)209-219
Number of pages11
JournalClinica Chimica Acta
Volume182
Issue number2
DOIs
Publication statusPublished - Jun 30 1989

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Keywords

  • Digestive cancer
  • Enzyme-linked immunosorbent assay (ELISA)
  • Human copper, zinc-superoxide dismutase (Cu, Zn-SOD)
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Oka, S., Ogino, K., Matsuura, S., Yoshimura, S., Yamamoto, K., Okazaki, Y., Takemoto, T., Kato, N., & Uda, T. (1989). Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer. Clinica Chimica Acta, 182(2), 209-219. https://doi.org/10.1016/0009-8981(89)90079-X