Human proton/oligopeptide transporter (POT) genes

Identification of putative human genes using bioinformatics

Christopher W. Botka, Thomas W. Wittig, Richard C. Graul, Garsten Uhd Nielsen, Wolfgang Sadée, Kazutaka Higaki, Gordon L. Amidon

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Purpose: The proton-dependent oligopeptide transporters (POT) gene family currently consists of ∼70 cloned cDNAs derived from diverse organisms. In mammals, two genes encoding peptide transporters, PepT1 and PepT2 have been cloned in several species including humans, in addition to a rat histidine/peptide transporter (rPHT1). Because the Candida elegans genome contains five putative POT genes, we searched the available protein and nucleic acid databases for additional mammalian/human POT genes, using iterative BLAST runs and the human expressed sequence tags (EST) database. The apparent human orthologue of rPHT1 (expression largely confined to rat brain and retina) was represented by numerous ESTs originating from many tissues. Assembly of these ESTs resulted in a contiguous sequence covering ∼95% of the suspected coding region. The contig sequences and analyses revealed the presence of several possible splice variants of hPHT1. A second closely related human EST-contig displayed high identity to a recently cloned mouse cDNA encoding cyclic adenosine monophosphate (cAMP)-inducible 1 protein (gi:4580995). This contig served to identify a PAC clone containing deduced exons and introns of the likely human orthologue (termed hPHT2). Northern analyses with EST clones indicated that hPHT1 is primarily expressed in skeletal muscle and spleen, whereas hPHT2 is found in spleen, placenta, lung, leukocytes, and heart. These results suggest considerable complexity of the human POT gene family, with relevance to the absorption and distribution of cephalosporins and other peptoid drugs.

Original languageEnglish
Pages (from-to)1-22
Number of pages22
JournalAAPS PharmSci
Volume2
Issue number2
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Oligopeptides
Forensic Anthropology
Computational Biology
Protons
Expressed Sequence Tags
Genes
Peptoids
Spleen
Complementary DNA
Clone Cells
Nucleic Acid Databases
Cephalosporins
Candida
Histidine
Cyclic AMP
Introns
Placenta
Sequence Analysis
Retina
Mammals

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Botka, C. W., Wittig, T. W., Graul, R. C., Nielsen, G. U., Sadée, W., Higaki, K., & Amidon, G. L. (2000). Human proton/oligopeptide transporter (POT) genes: Identification of putative human genes using bioinformatics. AAPS PharmSci, 2(2), 1-22.

Human proton/oligopeptide transporter (POT) genes : Identification of putative human genes using bioinformatics. / Botka, Christopher W.; Wittig, Thomas W.; Graul, Richard C.; Nielsen, Garsten Uhd; Sadée, Wolfgang; Higaki, Kazutaka; Amidon, Gordon L.

In: AAPS PharmSci, Vol. 2, No. 2, 2000, p. 1-22.

Research output: Contribution to journalArticle

Botka, CW, Wittig, TW, Graul, RC, Nielsen, GU, Sadée, W, Higaki, K & Amidon, GL 2000, 'Human proton/oligopeptide transporter (POT) genes: Identification of putative human genes using bioinformatics', AAPS PharmSci, vol. 2, no. 2, pp. 1-22.
Botka, Christopher W. ; Wittig, Thomas W. ; Graul, Richard C. ; Nielsen, Garsten Uhd ; Sadée, Wolfgang ; Higaki, Kazutaka ; Amidon, Gordon L. / Human proton/oligopeptide transporter (POT) genes : Identification of putative human genes using bioinformatics. In: AAPS PharmSci. 2000 ; Vol. 2, No. 2. pp. 1-22.
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