Human papillomavirus genome integration in multifocal vulvar Bowen's disease and squamous cell carcinoma

G. Nakanishi, K. Fujii, K. Asagoe, T. Tanaka, K. Iwatsuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human papillomavirus (HPV) is an important aetiological agent in cervical carcinomas and in malignant skin tumours. Integration of the HPV DNA into host genome is one of the most important risk factors for malignant transformation. We report a patient with multiple black plaques and an erythematous nodule on her vulva. On histological examination, multifocal vulvar Bowen's disease (BD) and invasive squamous cell carcinoma (SCC) were found. An amplification of papillomavirus oncogene transcripts (APOT) assay showed that two locations of BD had only episome-derived HPV16 transcripts, but the other two sites of BD and the nodule of invasive SCC had HPV16 transcripts derived from integration. Sequencing analysis revealed that the invasive SCC had its integration site at 8q24, the Myc locus. Our results suggest that the APOT assay in multiple sites of the same patient may be a valuable tool for evaluation of the clinical degree of malignancy for vulvar BD.

Original languageEnglish
JournalClinical and Experimental Dermatology
Volume34
Issue number8
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Vulvar Diseases
Bowen's Disease
Human Genome
Squamous Cell Carcinoma
Oncogenes
Vulva
Neoplasms
Plasmids
Genome
Carcinoma
Skin
DNA

ASJC Scopus subject areas

  • Dermatology

Cite this

Human papillomavirus genome integration in multifocal vulvar Bowen's disease and squamous cell carcinoma. / Nakanishi, G.; Fujii, K.; Asagoe, K.; Tanaka, T.; Iwatsuki, K.

In: Clinical and Experimental Dermatology, Vol. 34, No. 8, 12.2009.

Research output: Contribution to journalArticle

Nakanishi, G. ; Fujii, K. ; Asagoe, K. ; Tanaka, T. ; Iwatsuki, K. / Human papillomavirus genome integration in multifocal vulvar Bowen's disease and squamous cell carcinoma. In: Clinical and Experimental Dermatology. 2009 ; Vol. 34, No. 8.
@article{942c0da4147c4c1d9430506c75d16f9c,
title = "Human papillomavirus genome integration in multifocal vulvar Bowen's disease and squamous cell carcinoma",
abstract = "Human papillomavirus (HPV) is an important aetiological agent in cervical carcinomas and in malignant skin tumours. Integration of the HPV DNA into host genome is one of the most important risk factors for malignant transformation. We report a patient with multiple black plaques and an erythematous nodule on her vulva. On histological examination, multifocal vulvar Bowen's disease (BD) and invasive squamous cell carcinoma (SCC) were found. An amplification of papillomavirus oncogene transcripts (APOT) assay showed that two locations of BD had only episome-derived HPV16 transcripts, but the other two sites of BD and the nodule of invasive SCC had HPV16 transcripts derived from integration. Sequencing analysis revealed that the invasive SCC had its integration site at 8q24, the Myc locus. Our results suggest that the APOT assay in multiple sites of the same patient may be a valuable tool for evaluation of the clinical degree of malignancy for vulvar BD.",
author = "G. Nakanishi and K. Fujii and K. Asagoe and T. Tanaka and K. Iwatsuki",
year = "2009",
month = "12",
doi = "10.1111/j.1365-2230.2009.03708.x",
language = "English",
volume = "34",
journal = "Clinical and Experimental Dermatology",
issn = "0307-6938",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Human papillomavirus genome integration in multifocal vulvar Bowen's disease and squamous cell carcinoma

AU - Nakanishi, G.

AU - Fujii, K.

AU - Asagoe, K.

AU - Tanaka, T.

AU - Iwatsuki, K.

PY - 2009/12

Y1 - 2009/12

N2 - Human papillomavirus (HPV) is an important aetiological agent in cervical carcinomas and in malignant skin tumours. Integration of the HPV DNA into host genome is one of the most important risk factors for malignant transformation. We report a patient with multiple black plaques and an erythematous nodule on her vulva. On histological examination, multifocal vulvar Bowen's disease (BD) and invasive squamous cell carcinoma (SCC) were found. An amplification of papillomavirus oncogene transcripts (APOT) assay showed that two locations of BD had only episome-derived HPV16 transcripts, but the other two sites of BD and the nodule of invasive SCC had HPV16 transcripts derived from integration. Sequencing analysis revealed that the invasive SCC had its integration site at 8q24, the Myc locus. Our results suggest that the APOT assay in multiple sites of the same patient may be a valuable tool for evaluation of the clinical degree of malignancy for vulvar BD.

AB - Human papillomavirus (HPV) is an important aetiological agent in cervical carcinomas and in malignant skin tumours. Integration of the HPV DNA into host genome is one of the most important risk factors for malignant transformation. We report a patient with multiple black plaques and an erythematous nodule on her vulva. On histological examination, multifocal vulvar Bowen's disease (BD) and invasive squamous cell carcinoma (SCC) were found. An amplification of papillomavirus oncogene transcripts (APOT) assay showed that two locations of BD had only episome-derived HPV16 transcripts, but the other two sites of BD and the nodule of invasive SCC had HPV16 transcripts derived from integration. Sequencing analysis revealed that the invasive SCC had its integration site at 8q24, the Myc locus. Our results suggest that the APOT assay in multiple sites of the same patient may be a valuable tool for evaluation of the clinical degree of malignancy for vulvar BD.

UR - http://www.scopus.com/inward/record.url?scp=72549102912&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72549102912&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2230.2009.03708.x

DO - 10.1111/j.1365-2230.2009.03708.x

M3 - Article

C2 - 20055874

AN - SCOPUS:72549102912

VL - 34

JO - Clinical and Experimental Dermatology

JF - Clinical and Experimental Dermatology

SN - 0307-6938

IS - 8

ER -