TY - JOUR
T1 - Human monocyte chemoattractant protein-1 (MCP-1) Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE
AU - Yoshimura, Teizo
AU - Yuhki, Naoya
AU - Moore, Stephen K.
AU - Appella, Ettore
AU - Lerman, Michael I.
AU - Leonard, Edward J.
N1 - Funding Information:
AcknowledgementT: .Y. was supported by the Janapese OverseasC ancerF ellowshipo f the Foundationf or Promotion of CancerR esearch.
PY - 1989/2/27
Y1 - 1989/2/27
N2 - The purpose of this work was to analyze cDNA encoding human monocyte chemoattractant protein-1 (MCP-1), previously isolated from glioma cell line culture fluid. Screening of a cDNA library from total poly(A) RNA of glioma cell line U-105MG yielded a clone that coded for the entire MCP-1. Nucleotide sequence analysis and comparison with the amino acid sequence of purified MCP-1 showed that the cDNA clone comprises a 53-nucleotide 5′-non-coding region, an open reading frame coding for a 99-residue protein of which the last 76 residues correspond exactly to pure MCP-1, and a 389-nucleotide 3′-untranslated region. The hydrophobicity of the first 23 residues is typical of a signal peptide. Southern blot analysis of human and animal genomic DNA showed that there is a single MCP-1 gene, which is conserved in several primates. MCP-1 mRNA was induced in human peripheral blood mononuclear leukocytes (PBMNLs) by PHA, LPS and IL-1, but not by IL-2, TNF, or IFN-γ. Among proteins with similar sequences, the coding regions of MCP-1 and mouse JE show 68% identity. This suggests that MCP-1 is the human homologue of the mouse competence gene JE.
AB - The purpose of this work was to analyze cDNA encoding human monocyte chemoattractant protein-1 (MCP-1), previously isolated from glioma cell line culture fluid. Screening of a cDNA library from total poly(A) RNA of glioma cell line U-105MG yielded a clone that coded for the entire MCP-1. Nucleotide sequence analysis and comparison with the amino acid sequence of purified MCP-1 showed that the cDNA clone comprises a 53-nucleotide 5′-non-coding region, an open reading frame coding for a 99-residue protein of which the last 76 residues correspond exactly to pure MCP-1, and a 389-nucleotide 3′-untranslated region. The hydrophobicity of the first 23 residues is typical of a signal peptide. Southern blot analysis of human and animal genomic DNA showed that there is a single MCP-1 gene, which is conserved in several primates. MCP-1 mRNA was induced in human peripheral blood mononuclear leukocytes (PBMNLs) by PHA, LPS and IL-1, but not by IL-2, TNF, or IFN-γ. Among proteins with similar sequences, the coding regions of MCP-1 and mouse JE show 68% identity. This suggests that MCP-1 is the human homologue of the mouse competence gene JE.
KW - Chemotaxis
KW - Inflammation
KW - Macrophage infiltration
UR - http://www.scopus.com/inward/record.url?scp=0024505456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024505456&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(89)80590-3
DO - 10.1016/0014-5793(89)80590-3
M3 - Article
C2 - 2465924
AN - SCOPUS:0024505456
SN - 0014-5793
VL - 244
SP - 487
EP - 493
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -