Human identification from forensic materials by amplification of a human-specific sequence in the myoglobin gene

Toshiaki Ono, Satoru Miyaishi, Yuji Yamamoto, Kei Yoshitome, Takaki Ishikawa, Hideo Ishizu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 °C for 6 months, and from bloodstains heated at 150°C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.

Original languageEnglish
Pages (from-to)175-184
Number of pages10
JournalActa medica Okayama
Volume55
Issue number3
Publication statusPublished - Dec 1 2001

Keywords

  • Myoglobin
  • Polymerase chain reaction
  • Species identification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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