Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor

T. Takebe, S. Kobayashi, H. Kan, H. Suzuki, Y. Yabuki, M. Mizuno, T. Adegawa, Tomohiko Yoshioka, J. Tanaka, J. Maegawa, H. Taniguchi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Transplantation of bioengineered elastic cartilage is considered to be a promising approach for patients with craniofacial defects. We have previously shown that human ear perichondrium harbors a population of cartilage progenitor cells (CPCs). The aim of this study was to examine the use of a rotating wall vessel (RWV) bioreactor for CPCs to engineer 3-D elastic cartilage in vitro. Human CPCs isolated from ear perichondrium were expanded and differentiated into chondrocytes under 2-D culture conditions. Fully differentiated CPCs were seeded into recently developed pC-HAp/ChS (porous material consisted of collagen, hydroxyapatite, and chondroitinsulfate) scaffolds and 3-D cultivated utilizing a RWV bioreactor. 3-D engineered constructs appeared shiny with a yellowish, cartilage-like morphology. The shape of the molded scaffold was maintained after RWV cultivation. Hematoxylin and eosin staining showed engraftment of CPCs inside pC-HAp/ChS. Alcian blue and Elastica Van Gieson staining showed of proteoglycan and elastic fibers, which are unique extracellular matrices of elastic cartilage. Thus, human CPCs formed elastic cartilage-like tissue after 3-D cultivation in a RWV bioreactor. These techniques may assist future efforts to reconstruct complicate structures composed of elastic cartilage in vitro.

Original languageEnglish
Pages (from-to)1158-1161
Number of pages4
JournalTransplantation Proceedings
Volume44
Issue number4
DOIs
Publication statusPublished - May 2012
Externally publishedYes

Fingerprint

Elastic Cartilage
Bioreactors
Cartilage
Stem Cells
Ear
Staining and Labeling
Alcian Blue
Elastic Tissue
Rubber
Proteoglycans
Durapatite
Hematoxylin
Eosine Yellowish-(YS)
Chondrocytes
Extracellular Matrix
Collagen
Transplantation

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor. / Takebe, T.; Kobayashi, S.; Kan, H.; Suzuki, H.; Yabuki, Y.; Mizuno, M.; Adegawa, T.; Yoshioka, Tomohiko; Tanaka, J.; Maegawa, J.; Taniguchi, H.

In: Transplantation Proceedings, Vol. 44, No. 4, 05.2012, p. 1158-1161.

Research output: Contribution to journalArticle

Takebe, T, Kobayashi, S, Kan, H, Suzuki, H, Yabuki, Y, Mizuno, M, Adegawa, T, Yoshioka, T, Tanaka, J, Maegawa, J & Taniguchi, H 2012, 'Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor', Transplantation Proceedings, vol. 44, no. 4, pp. 1158-1161. https://doi.org/10.1016/j.transproceed.2012.03.038
Takebe, T. ; Kobayashi, S. ; Kan, H. ; Suzuki, H. ; Yabuki, Y. ; Mizuno, M. ; Adegawa, T. ; Yoshioka, Tomohiko ; Tanaka, J. ; Maegawa, J. ; Taniguchi, H. / Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor. In: Transplantation Proceedings. 2012 ; Vol. 44, No. 4. pp. 1158-1161.
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