The distance information is very important to develop a virtual reality system with high reality performance. However, it is very difficult to realize a man-machine interface to present the distance information. Because the human perceptive distance is different from the actual distance, and also the spatial scale of the real world is different from the virtual world. In order to develop a distance man-machine interface with high reality performance, human characteristics on the distance perception are needed to be measured. In our. previous study, the characteristics of human distance perception of the upper limbs were measured. The results suggested that the human perceptive distance was different from the actual distance. And, a model of distance perception was also proposed. In this study, the authors measured characteristics of human distance perception of the lower limbs with walk condition. The experimental results suggested that the human perceptive distance of the lower limbs is also different from the actual distance. The experimental results provide useful basic data to design the distance manmachine interface of the virtual reality systems, and to investigate human mechanism of the distance perception.