TY - JOUR
T1 - Human colon cancer cells express the functional Fas ligand
AU - Ding, Er Xun
AU - Hizuta, Akio
AU - Morimoto, Yoshinori
AU - Tanida, Tohru
AU - Hongo, Toshie
AU - Ishii, Tatsuaki
AU - Yamano, Taketoshi
AU - Fujiwara, Toshiyoshi
AU - Iwagaki, Hiromi
AU - Tanaka, Noriaki
PY - 1998/7/1
Y1 - 1998/7/1
N2 - Fas ligand (FasL) belongs to the TNF superfamily. It is induced in activated lymphocytes and eliminates Fas-positive lymphocytes, resulting in the down-regulation of immune responses. FasL has also been detected in tissues other than lymphoid cells. We investigated the expression and function of FasL on human colon cancer cells. FasL mRNA was detected by RT- PCR in all six colon cancer cell lines tested and was not found on fibroblasts. FasL protein was detected in DLD-1, LoVo, HCT-116 and RPMI 4788 cells by immunohistochemical staining. DLD-1, LoVo and WiDr were cytotoxic against mouse T lymphoma cells which were transfected with human Fas receptor cDNA. The cytotoxicity was significantly enhanced by phorbol 12-myristate 13- acetate (PMA) and ionomycin. Our data suggest that the FasL expressed in human colon cancer cells may be regulated by endogeneous factors in the microenvironment of the host and facilitates the escape from the host immune system.
AB - Fas ligand (FasL) belongs to the TNF superfamily. It is induced in activated lymphocytes and eliminates Fas-positive lymphocytes, resulting in the down-regulation of immune responses. FasL has also been detected in tissues other than lymphoid cells. We investigated the expression and function of FasL on human colon cancer cells. FasL mRNA was detected by RT- PCR in all six colon cancer cell lines tested and was not found on fibroblasts. FasL protein was detected in DLD-1, LoVo, HCT-116 and RPMI 4788 cells by immunohistochemical staining. DLD-1, LoVo and WiDr were cytotoxic against mouse T lymphoma cells which were transfected with human Fas receptor cDNA. The cytotoxicity was significantly enhanced by phorbol 12-myristate 13- acetate (PMA) and ionomycin. Our data suggest that the FasL expressed in human colon cancer cells may be regulated by endogeneous factors in the microenvironment of the host and facilitates the escape from the host immune system.
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M3 - Article
C2 - 9755840
AN - SCOPUS:0031660412
SN - 1078-0297
VL - 101
SP - 13
EP - 24
JO - Research Communications in Molecular Pathology and Pharmacology
JF - Research Communications in Molecular Pathology and Pharmacology
IS - 1
ER -
