Human chondrosarcoma secretes vascular endothelial growth factor to induce tumor angiogenesis and stores basic fibroblast growth factor for regulation of its own growth

Takayuki Furumatsu, Keiichiro Nishida, Akira Kawai, Masayoshi Namba, Hajime Inoue, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are well-known factors that induce neovascularization in many tumors. The molecular mechanisms that regulate tumor angiogenesis in human chondrosarcoma are not clear. We assessed in this work the angiogenic activities of a human chondrosarcoma cell line (OUMS-27) in vivo and determined the efficacies of angiogenic factors derived from OUMS-27 cells on human umbilical vein endothelial cells (HUVECs) in vitro. Tumor xenografts induced an increase in the formation of neovessels, but the distributions of Ki-67 antigen, VEGF and bFGF were unaffected. We also demonstrated that OUMS-27 cells secreted VEGF165 into the culture medium and that it was the maximal angiogenic factor to stimulate endothelial proliferation and migration in chondrosarcoma. Anti-VEGF antibodies induced an approximately 70% inhibition of these responses of HUVECs, but did not have any effect on OUMS-27 cells. Anti-bFGF antibodies suppressed not only the activities of HUVECs but also the growth of tumor cells in vitro. We indicate that angiogenesis is principally elicited by VEGF165 and that tumorigenesis is mainly regulated by bFGF stored in the extracellular matrix of OUMS-27 cells. The present study may offer the availability of combination therapies for inhibition of VEGF and bFGF action on vascular endothelial cells and chondrosarcoma cells, respectively.

Original languageEnglish
Pages (from-to)313-322
Number of pages10
JournalInternational Journal of Cancer
Volume97
Issue number3
DOIs
Publication statusPublished - Jan 20 2002

Fingerprint

Chondrosarcoma
Fibroblast Growth Factor 2
Vascular Endothelial Growth Factor A
Human Umbilical Vein Endothelial Cells
Growth
Neoplasms
Angiogenesis Inducing Agents
Ki-67 Antigen
Antibodies
Heterografts
Human Activities
Extracellular Matrix
Culture Media
Carcinogenesis
Endothelial Cells
Cell Line

Keywords

  • Basic fibroblast growth factor
  • Chondrosarcoma
  • Endothelial cell
  • Transforming growth factor
  • Tumor angiogenesis
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Human chondrosarcoma secretes vascular endothelial growth factor to induce tumor angiogenesis and stores basic fibroblast growth factor for regulation of its own growth. / Furumatsu, Takayuki; Nishida, Keiichiro; Kawai, Akira; Namba, Masayoshi; Inoue, Hajime; Ninomiya, Yoshifumi.

In: International Journal of Cancer, Vol. 97, No. 3, 20.01.2002, p. 313-322.

Research output: Contribution to journalArticle

@article{cc7bf0ea42154090880ab897991e6e99,
title = "Human chondrosarcoma secretes vascular endothelial growth factor to induce tumor angiogenesis and stores basic fibroblast growth factor for regulation of its own growth",
abstract = "Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are well-known factors that induce neovascularization in many tumors. The molecular mechanisms that regulate tumor angiogenesis in human chondrosarcoma are not clear. We assessed in this work the angiogenic activities of a human chondrosarcoma cell line (OUMS-27) in vivo and determined the efficacies of angiogenic factors derived from OUMS-27 cells on human umbilical vein endothelial cells (HUVECs) in vitro. Tumor xenografts induced an increase in the formation of neovessels, but the distributions of Ki-67 antigen, VEGF and bFGF were unaffected. We also demonstrated that OUMS-27 cells secreted VEGF165 into the culture medium and that it was the maximal angiogenic factor to stimulate endothelial proliferation and migration in chondrosarcoma. Anti-VEGF antibodies induced an approximately 70{\%} inhibition of these responses of HUVECs, but did not have any effect on OUMS-27 cells. Anti-bFGF antibodies suppressed not only the activities of HUVECs but also the growth of tumor cells in vitro. We indicate that angiogenesis is principally elicited by VEGF165 and that tumorigenesis is mainly regulated by bFGF stored in the extracellular matrix of OUMS-27 cells. The present study may offer the availability of combination therapies for inhibition of VEGF and bFGF action on vascular endothelial cells and chondrosarcoma cells, respectively.",
keywords = "Basic fibroblast growth factor, Chondrosarcoma, Endothelial cell, Transforming growth factor, Tumor angiogenesis, Vascular endothelial growth factor",
author = "Takayuki Furumatsu and Keiichiro Nishida and Akira Kawai and Masayoshi Namba and Hajime Inoue and Yoshifumi Ninomiya",
year = "2002",
month = "1",
day = "20",
doi = "10.1002/ijc.1607",
language = "English",
volume = "97",
pages = "313--322",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Human chondrosarcoma secretes vascular endothelial growth factor to induce tumor angiogenesis and stores basic fibroblast growth factor for regulation of its own growth

AU - Furumatsu, Takayuki

AU - Nishida, Keiichiro

AU - Kawai, Akira

AU - Namba, Masayoshi

AU - Inoue, Hajime

AU - Ninomiya, Yoshifumi

PY - 2002/1/20

Y1 - 2002/1/20

N2 - Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are well-known factors that induce neovascularization in many tumors. The molecular mechanisms that regulate tumor angiogenesis in human chondrosarcoma are not clear. We assessed in this work the angiogenic activities of a human chondrosarcoma cell line (OUMS-27) in vivo and determined the efficacies of angiogenic factors derived from OUMS-27 cells on human umbilical vein endothelial cells (HUVECs) in vitro. Tumor xenografts induced an increase in the formation of neovessels, but the distributions of Ki-67 antigen, VEGF and bFGF were unaffected. We also demonstrated that OUMS-27 cells secreted VEGF165 into the culture medium and that it was the maximal angiogenic factor to stimulate endothelial proliferation and migration in chondrosarcoma. Anti-VEGF antibodies induced an approximately 70% inhibition of these responses of HUVECs, but did not have any effect on OUMS-27 cells. Anti-bFGF antibodies suppressed not only the activities of HUVECs but also the growth of tumor cells in vitro. We indicate that angiogenesis is principally elicited by VEGF165 and that tumorigenesis is mainly regulated by bFGF stored in the extracellular matrix of OUMS-27 cells. The present study may offer the availability of combination therapies for inhibition of VEGF and bFGF action on vascular endothelial cells and chondrosarcoma cells, respectively.

AB - Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are well-known factors that induce neovascularization in many tumors. The molecular mechanisms that regulate tumor angiogenesis in human chondrosarcoma are not clear. We assessed in this work the angiogenic activities of a human chondrosarcoma cell line (OUMS-27) in vivo and determined the efficacies of angiogenic factors derived from OUMS-27 cells on human umbilical vein endothelial cells (HUVECs) in vitro. Tumor xenografts induced an increase in the formation of neovessels, but the distributions of Ki-67 antigen, VEGF and bFGF were unaffected. We also demonstrated that OUMS-27 cells secreted VEGF165 into the culture medium and that it was the maximal angiogenic factor to stimulate endothelial proliferation and migration in chondrosarcoma. Anti-VEGF antibodies induced an approximately 70% inhibition of these responses of HUVECs, but did not have any effect on OUMS-27 cells. Anti-bFGF antibodies suppressed not only the activities of HUVECs but also the growth of tumor cells in vitro. We indicate that angiogenesis is principally elicited by VEGF165 and that tumorigenesis is mainly regulated by bFGF stored in the extracellular matrix of OUMS-27 cells. The present study may offer the availability of combination therapies for inhibition of VEGF and bFGF action on vascular endothelial cells and chondrosarcoma cells, respectively.

KW - Basic fibroblast growth factor

KW - Chondrosarcoma

KW - Endothelial cell

KW - Transforming growth factor

KW - Tumor angiogenesis

KW - Vascular endothelial growth factor

UR - http://www.scopus.com/inward/record.url?scp=0037137897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037137897&partnerID=8YFLogxK

U2 - 10.1002/ijc.1607

DO - 10.1002/ijc.1607

M3 - Article

VL - 97

SP - 313

EP - 322

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 3

ER -