Human annexin V binds to sulfatide

Contribution of regulation of blood coagulation

Michiru Ida, Ayano Satoh, Isamu Matsumoto, Kyoko Kojima-Aikawa

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Annexin V is a calcium-dependent phospholipid-binding protein that exhibits anticoagulant activity on binding to phosphatidylserine exposed on the activated surfaces of endothelial cells and platelets, inhibiting activation of factor X and prothrombin in the blood coagulation cascade. Sulfatide (galactosylecramide I3-sulfate), one of the glycosphingolipids of the platelet cell membrane, is thought to be involved in blood coagulation systems via activation of factor XII. In this study, we examined whether or not annexin V binds to sulfatide and affects the coagulant activity of sulfatide. Solid phase assaying of annexin V revealed that it binds specifically to sulfatide, i.e. not to galactosylceramide or gangliosides, in the presence of calcium ions. Affinity analysis by means of surface plasmon resonance showed that the K D of the interaction between annexin V and sulfatide is 1.2 μM. Kinetic turbidometric assaying of plasma coagulation initiated by CaCl 2 revealed that the coagulation rate in the presence of sulfatide or phosphatidylserine was decreased by annexin V. These results suggest that annexin V regulates coagulability in the blood stream by binding not only to phosphatidylserine but also to sulfatide.

Original languageEnglish
Pages (from-to)583-588
Number of pages6
JournalJournal of Biochemistry
Volume135
Issue number5
DOIs
Publication statusPublished - May 2004
Externally publishedYes

Fingerprint

Sulfoglycosphingolipids
Annexin A5
Blood Coagulation
Coagulation
Blood
Phosphatidylserines
Platelets
Chemical activation
Galactosylceramides
Factor XII
Calcium
Factor X
Glycosphingolipids
Coagulants
Surface Plasmon Resonance
Gangliosides
Endothelial cells
Platelet Activation
Surface plasmon resonance
Prothrombin

Keywords

  • Annexin
  • Anticoagulant
  • Lectin
  • Plasma coagulation
  • Sulfatide

ASJC Scopus subject areas

  • Biochemistry

Cite this

Human annexin V binds to sulfatide : Contribution of regulation of blood coagulation. / Ida, Michiru; Satoh, Ayano; Matsumoto, Isamu; Kojima-Aikawa, Kyoko.

In: Journal of Biochemistry, Vol. 135, No. 5, 05.2004, p. 583-588.

Research output: Contribution to journalArticle

Ida, Michiru ; Satoh, Ayano ; Matsumoto, Isamu ; Kojima-Aikawa, Kyoko. / Human annexin V binds to sulfatide : Contribution of regulation of blood coagulation. In: Journal of Biochemistry. 2004 ; Vol. 135, No. 5. pp. 583-588.
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