Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice

Kenjiro Nagaoka, Keiki Ogino, Noriyoshi Ogino, Tatsuo Ito, Kei Takemoto, Shihona Ogino, Yuka Seki, Hiroki Hamada, Yoshihisa Fujikura

Research output: Contribution to journalArticle

Abstract

The synergic allergic inflammatory effects of particulate matter (PM) 2.5 and human albumin were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 or PM2.5 plus human albumin with aluminum oxide was injected twice intraperitoneally for sensitization. After 7 days, PM2.5 or PM2.5 plus human albumin was administered five times intranasally to mice for further sensitization. Subsequently, PM2.5 was administered as a challenge on the 11th day. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th 1 , Th 2 cytokines, chemokines, and mucus proteins (MUC5AC and MUC5B) in the lung tissue and histopathology. Although PM2.5 or human albumin alone did not induce allergic airway inflammation, simultaneous inoculation of PM2.5 and human albumin-induced airway inflammation showing increase in AHR, total BALF cell numbers, mRNA levels of IL-13, eotaxin 1, eotaxin 2, and MUC5AC, and anti-IG against human serum albumin. Inflammation was observed around the bronchus in PM2.5 plus human albumin-induced lungs. These results demonstrate that PM2.5 can induce allergic airway inflammation through the synergistic action with human albumin in NC/Nga mice.

Original languageEnglish
JournalEnvironmental Toxicology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Albumins
Inflammation
histopathology
fluid
mucus
mite
aluminum oxide
inoculation
particulate matter
serum
protein
Bronchoalveolar Lavage Fluid
Chemokine CCL24
Chemokine CCL11
Cell Count
Messenger RNA
Fluids
Interleukin-13
Particulate Matter
Aluminum Oxide

Keywords

  • allergic airway inflammation
  • human albumin
  • NC/Nga mice
  • PM2.5

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice. / Nagaoka, Kenjiro; Ogino, Keiki; Ogino, Noriyoshi; Ito, Tatsuo; Takemoto, Kei; Ogino, Shihona; Seki, Yuka; Hamada, Hiroki; Fujikura, Yoshihisa.

In: Environmental Toxicology, 01.01.2019.

Research output: Contribution to journalArticle

Nagaoka, K, Ogino, K, Ogino, N, Ito, T, Takemoto, K, Ogino, S, Seki, Y, Hamada, H & Fujikura, Y 2019, 'Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice', Environmental Toxicology. https://doi.org/10.1002/tox.22751
Nagaoka, Kenjiro ; Ogino, Keiki ; Ogino, Noriyoshi ; Ito, Tatsuo ; Takemoto, Kei ; Ogino, Shihona ; Seki, Yuka ; Hamada, Hiroki ; Fujikura, Yoshihisa. / Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice. In: Environmental Toxicology. 2019.
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