Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice

Kenjiro Nagaoka, Keiki Ogino, Noriyoshi Ogino, Tatsuo Ito, Kei Takemoto, Shihona Ogino, Yuka Seki, Hiroki Hamada, Yoshihisa Fujikura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The synergic allergic inflammatory effects of particulate matter (PM) 2.5 and human albumin were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 or PM2.5 plus human albumin with aluminum oxide was injected twice intraperitoneally for sensitization. After 7 days, PM2.5 or PM2.5 plus human albumin was administered five times intranasally to mice for further sensitization. Subsequently, PM2.5 was administered as a challenge on the 11th day. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th1, Th2 cytokines, chemokines, and mucus proteins (MUC5AC and MUC5B) in the lung tissue and histopathology. Although PM2.5 or human albumin alone did not induce allergic airway inflammation, simultaneous inoculation of PM2.5 and human albumin-induced airway inflammation showing increase in AHR, total BALF cell numbers, mRNA levels of IL-13, eotaxin 1, eotaxin 2, and MUC5AC, and anti-IG against human serum albumin. Inflammation was observed around the bronchus in PM2.5 plus human albumin-induced lungs. These results demonstrate that PM2.5 can induce allergic airway inflammation through the synergistic action with human albumin in NC/Nga mice.

Original languageEnglish
Pages (from-to)836-843
Number of pages8
JournalEnvironmental Toxicology
Volume34
Issue number7
DOIs
Publication statusPublished - Jul 2019

Keywords

  • NC/Nga mice
  • PM2.5
  • allergic airway inflammation
  • human albumin

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

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  • Cite this

    Nagaoka, K., Ogino, K., Ogino, N., Ito, T., Takemoto, K., Ogino, S., Seki, Y., Hamada, H., & Fujikura, Y. (2019). Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice. Environmental Toxicology, 34(7), 836-843. https://doi.org/10.1002/tox.22751