Human AIM-1: cDNA cloning and reduced expression during endomitosis in megakaryocyte-lineage cells

Hiroshi Katayama; Takahide Ota; Kimiko Morita; Yasuhiko Terada; Fumio Suzuki; Osamu Katoh; Masaaki Tatsuka

doi:10.1016/S0378-1119(98)00522-8

Human AIM-1: cDNA cloning and reduced expression during endomitosis in megakaryocyte-lineage cells

Hiroshi Katayama, Takahide Ota, Kimiko Morita, Yasuhiko Terada, Fumio Suzuki, Osamu Katoh, Masaaki Tatsuka

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The rat AIM-1 gene encoding an Aurora- and Ip11-like midbody-associated protein serine/threonine kinase has a mitotic regulator function playing a key role in the onset of cytokinesis during mitosis. This report presents a cDNA sequence and megakaryocytic differentiation-dependent expression profile of the human AIM-1 gene. The nucleotide sequences of the human AIM-1 were identified from cDNAs of three cell lines, including cervical Carcinoma HeLa cells, colorectal tumor SW480 cells, and normal human diploid skin fibroblast NHDF cells, and no mutation was found. The expression levels of AIM-1 transcript were markedly reduced during differentiation into megakaryocytic cell lineage in human leukemia cells induced by 12-o-tetradecanoylphorbol- 13-acetate (TPA), suggesting that the downregulation of AIM-1 contributes to the differentiation by repeated duplication of DNA without cytokinesis (endomitosis).

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Gene
Volume	224
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(98)00522-8
Publication status	Published - Dec 11 1998

Keywords

Cytokinesis
Midbody
Mitosis
Polyploidization
STK-1
Serine/threonine kinase

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(98)00522-8

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title = "Human AIM-1: cDNA cloning and reduced expression during endomitosis in megakaryocyte-lineage cells",

abstract = "The rat AIM-1 gene encoding an Aurora- and Ip11-like midbody-associated protein serine/threonine kinase has a mitotic regulator function playing a key role in the onset of cytokinesis during mitosis. This report presents a cDNA sequence and megakaryocytic differentiation-dependent expression profile of the human AIM-1 gene. The nucleotide sequences of the human AIM-1 were identified from cDNAs of three cell lines, including cervical Carcinoma HeLa cells, colorectal tumor SW480 cells, and normal human diploid skin fibroblast NHDF cells, and no mutation was found. The expression levels of AIM-1 transcript were markedly reduced during differentiation into megakaryocytic cell lineage in human leukemia cells induced by 12-o-tetradecanoylphorbol- 13-acetate (TPA), suggesting that the downregulation of AIM-1 contributes to the differentiation by repeated duplication of DNA without cytokinesis (endomitosis).",

keywords = "Cytokinesis, Midbody, Mitosis, Polyploidization, STK-1, Serine/threonine kinase",

author = "Hiroshi Katayama and Takahide Ota and Kimiko Morita and Yasuhiko Terada and Fumio Suzuki and Osamu Katoh and Masaaki Tatsuka",

note = "Funding Information: We thank H. Shimada for his encouragement to H.K. This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan (09248227, 10151234, 10480135, and 10151234), from the Japan Atomic Energy Research Institute under contract to the Nuclear Safety Research Associaiton, from the Electric Technology Research Foundation of Chugoku in Japan, and from the Kanazawa Medical University High-Technology Center.",

year = "1998",

month = dec,

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doi = "10.1016/S0378-1119(98)00522-8",

language = "English",

volume = "224",

pages = "1--7",

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T1 - Human AIM-1

T2 - cDNA cloning and reduced expression during endomitosis in megakaryocyte-lineage cells

AU - Katayama, Hiroshi

AU - Ota, Takahide

AU - Morita, Kimiko

AU - Terada, Yasuhiko

AU - Suzuki, Fumio

AU - Katoh, Osamu

AU - Tatsuka, Masaaki

N1 - Funding Information: We thank H. Shimada for his encouragement to H.K. This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan (09248227, 10151234, 10480135, and 10151234), from the Japan Atomic Energy Research Institute under contract to the Nuclear Safety Research Associaiton, from the Electric Technology Research Foundation of Chugoku in Japan, and from the Kanazawa Medical University High-Technology Center.

PY - 1998/12/11

Y1 - 1998/12/11

N2 - The rat AIM-1 gene encoding an Aurora- and Ip11-like midbody-associated protein serine/threonine kinase has a mitotic regulator function playing a key role in the onset of cytokinesis during mitosis. This report presents a cDNA sequence and megakaryocytic differentiation-dependent expression profile of the human AIM-1 gene. The nucleotide sequences of the human AIM-1 were identified from cDNAs of three cell lines, including cervical Carcinoma HeLa cells, colorectal tumor SW480 cells, and normal human diploid skin fibroblast NHDF cells, and no mutation was found. The expression levels of AIM-1 transcript were markedly reduced during differentiation into megakaryocytic cell lineage in human leukemia cells induced by 12-o-tetradecanoylphorbol- 13-acetate (TPA), suggesting that the downregulation of AIM-1 contributes to the differentiation by repeated duplication of DNA without cytokinesis (endomitosis).

AB - The rat AIM-1 gene encoding an Aurora- and Ip11-like midbody-associated protein serine/threonine kinase has a mitotic regulator function playing a key role in the onset of cytokinesis during mitosis. This report presents a cDNA sequence and megakaryocytic differentiation-dependent expression profile of the human AIM-1 gene. The nucleotide sequences of the human AIM-1 were identified from cDNAs of three cell lines, including cervical Carcinoma HeLa cells, colorectal tumor SW480 cells, and normal human diploid skin fibroblast NHDF cells, and no mutation was found. The expression levels of AIM-1 transcript were markedly reduced during differentiation into megakaryocytic cell lineage in human leukemia cells induced by 12-o-tetradecanoylphorbol- 13-acetate (TPA), suggesting that the downregulation of AIM-1 contributes to the differentiation by repeated duplication of DNA without cytokinesis (endomitosis).

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KW - Midbody

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KW - Polyploidization

KW - STK-1

KW - Serine/threonine kinase

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Human AIM-1: cDNA cloning and reduced expression during endomitosis in megakaryocyte-lineage cells

Abstract

Keywords

ASJC Scopus subject areas

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