Abstract
The purpose of this study was to demonstrate the immunohistochemical localization and distribution of human α-and β-defensins, peptides with antimicrobial activity, in oral mucoepidermoid carcinoma tissue. Tissue samples were embedded in paraffin and α- and β-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells that constituted the ducts, as well as neutrophils, were positively immunostained with the anti-α-defensin antibody (HNPs). On the other hand, epidermoid cells and intermediate cells were intensely stained with the anti-β-defensin-2 (HBD-2) antibody. Mucous-secreting cells were clearly not immunostained with the anti-HBD-2 antibody. The epithelial hyperplasia region adjacent to the tumor tissues was also positively immunostained with the anti-HBD-2 antibody.
| Original language | English |
|---|---|
| Pages (from-to) | 2171-2174 |
| Number of pages | 4 |
| Journal | Anticancer Research |
| Volume | 21 |
| Issue number | 3 C |
| Publication status | Published - 2001 |
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Keywords
- β-defensin
- Defensin
- HBD-2
- Mucoepidermoid carcinoma
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Human α-and β-defensin immunoreactivity in oral mucoepidermoid carcinomas. / Mizukawa, Nobuyoshi; Sawaki, K.; Nagatsuka, Hitoshi; Kamio, M.; Yamachika, Eiki; Fukunaga, J.; Ueno, T.; Takagi, S.; Sugahara, T.
In: Anticancer Research, Vol. 21, No. 3 C, 2001, p. 2171-2174.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human α-and β-defensin immunoreactivity in oral mucoepidermoid carcinomas
AU - Mizukawa, Nobuyoshi
AU - Sawaki, K.
AU - Nagatsuka, Hitoshi
AU - Kamio, M.
AU - Yamachika, Eiki
AU - Fukunaga, J.
AU - Ueno, T.
AU - Takagi, S.
AU - Sugahara, T.
PY - 2001
Y1 - 2001
N2 - The purpose of this study was to demonstrate the immunohistochemical localization and distribution of human α-and β-defensins, peptides with antimicrobial activity, in oral mucoepidermoid carcinoma tissue. Tissue samples were embedded in paraffin and α- and β-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells that constituted the ducts, as well as neutrophils, were positively immunostained with the anti-α-defensin antibody (HNPs). On the other hand, epidermoid cells and intermediate cells were intensely stained with the anti-β-defensin-2 (HBD-2) antibody. Mucous-secreting cells were clearly not immunostained with the anti-HBD-2 antibody. The epithelial hyperplasia region adjacent to the tumor tissues was also positively immunostained with the anti-HBD-2 antibody.
AB - The purpose of this study was to demonstrate the immunohistochemical localization and distribution of human α-and β-defensins, peptides with antimicrobial activity, in oral mucoepidermoid carcinoma tissue. Tissue samples were embedded in paraffin and α- and β-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells that constituted the ducts, as well as neutrophils, were positively immunostained with the anti-α-defensin antibody (HNPs). On the other hand, epidermoid cells and intermediate cells were intensely stained with the anti-β-defensin-2 (HBD-2) antibody. Mucous-secreting cells were clearly not immunostained with the anti-HBD-2 antibody. The epithelial hyperplasia region adjacent to the tumor tissues was also positively immunostained with the anti-HBD-2 antibody.
KW - β-defensin
KW - Defensin
KW - HBD-2
KW - Mucoepidermoid carcinoma
UR - http://www.scopus.com/inward/record.url?scp=0034903080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034903080&partnerID=8YFLogxK
M3 - Article
C2 - 11501842
AN - SCOPUS:0034903080
VL - 21
SP - 2171
EP - 2174
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 3 C
ER -