H+-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles

Yoshinor Moriyama, Masamitsu Futai

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H+-ATPase by immunoblotting with antibodies against H+-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H+-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.

Original languageEnglish
Pages (from-to)443-448
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume173
Issue number1
DOIs
Publication statusPublished - Nov 30 1990

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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