TY - JOUR
T1 - H+-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles
AU - Moriyama, Yoshinor
AU - Futai, Masamitsu
N1 - Funding Information:
Acknowledgnents. This work was supported in part by grants from the Ministry of Education, Science, and Culture of Japan, a grant for a Research Program on "Creation of New Materials through Intelligent Design" of ISIR, Osaka University, and a Human Frontier Science Program Research Grant.
PY - 1990/11/30
Y1 - 1990/11/30
N2 - Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H+-ATPase by immunoblotting with antibodies against H+-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H+-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.
AB - Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H+-ATPase by immunoblotting with antibodies against H+-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H+-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.
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U2 - 10.1016/S0006-291X(05)81078-2
DO - 10.1016/S0006-291X(05)81078-2
M3 - Article
C2 - 1979489
AN - SCOPUS:0025675440
VL - 173
SP - 443
EP - 448
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -