H+-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles

Yoshinor Moriyama; Masamitsu Futai

doi:10.1016/S0006-291X(05)81078-2

H⁺-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles

Yoshinor Moriyama, Masamitsu Futai

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

85 Citations (Scopus)

Abstract

Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H⁺ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H⁺-ATPase by immunoblotting with antibodies against H⁺-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H⁺-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.

Original language	English
Pages (from-to)	443-448
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	173
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(05)81078-2
Publication status	Published - Nov 30 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(05)81078-2

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title = "H+-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles",

abstract = "Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H+-ATPase by immunoblotting with antibodies against H+-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H+-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.",

author = "Yoshinor Moriyama and Masamitsu Futai",

note = "Funding Information: Acknowledgnents. This work was supported in part by grants from the Ministry of Education, Science, and Culture of Japan, a grant for a Research Program on {"}Creation of New Materials through Intelligent Design{"} of ISIR, Osaka University, and a Human Frontier Science Program Research Grant.",

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T1 - H+-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles

AU - Moriyama, Yoshinor

AU - Futai, Masamitsu

N1 - Funding Information: Acknowledgnents. This work was supported in part by grants from the Ministry of Education, Science, and Culture of Japan, a grant for a Research Program on "Creation of New Materials through Intelligent Design" of ISIR, Osaka University, and a Human Frontier Science Program Research Grant.

PY - 1990/11/30

Y1 - 1990/11/30

N2 - Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H+-ATPase by immunoblotting with antibodies against H+-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H+-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.

AB - Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H+-ATPase by immunoblotting with antibodies against H+-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H+-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.

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H⁺-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles

Abstract

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