Abstract
Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15 % of the total protein, were identified as subunits of H+-ATPase by immunoblotting with antibodies against H+-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and γ-aminobutyrate by the synaptic vesicles. These results indicated that H+-ATPase is a major constituent of the vesicles (consisting about 20 % of their total protein) and is a primary pump for accumulation of neurotransmitters.
| Original language | English |
|---|---|
| Pages (from-to) | 443-448 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 173 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Nov 30 1990 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Moriyama, Y., & Futai, M. (1990). H+-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles. Biochemical and Biophysical Research Communications, 173(1), 443-448. https://doi.org/10.1016/S0006-291X(05)81078-2