HSP90α deficiency does not affect immunoglobulin gene hypermutation and class switch but causes enhanced MHC class II antigen presentation

Yingqian Li, Shuyin Li, Mari Hoshino, Rikiya Ishikawa, Chiaki Kajiwara, Xiang Gao, Yaofeng Zhao, Satoshi Ishido, Heiichiro Udono, Ji Yang Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Heat shock protein 90 (HSP90) is a molecular chaperone required for efficient antigen presentation and cross-presentation. In addition, HSP90 was recently reported to interact with and stabilize the activation-induced cytidine deaminase (AID) and plays a critical role in immunoglobulin gene hypermutation and class switch recombination. In mice and humans, there are two HSP90 isoforms, HSP90α and HSP90β, but the in vivo role of each isoform remains largely unknown. Here we have analyzed humoral immune responses in HSP90α-deficient mice. We found that HSP90α deficiency did not affect AID protein expression. B cell development and maturation, as well as immunoglobulin gene hypermuation and class switch, occurred normally in HSP90α-deficient mice. However, antibody production to a T-dependent antigen was elevated in the mutant mice and this was associated with enhanced MHC class II antigen presentation to T helper cells by dendritic cells. Our results reveal a previously unidentified inhibitory role for HSP90α isoform in MHC class II antigen presentation and the humoral immune response. Along with our recent finding that HSP90α is required for antigen cross-presentation, these results suggest that HSP90α controls the balance of humoral and cellular immunity by dictating the fate of presentation of exogenous antigen.

Original languageEnglish
Article numberdxs076
Pages (from-to)751-758
Number of pages8
JournalInternational Immunology
Volume24
Issue number12
DOIs
Publication statusPublished - Dec 1 2012
Externally publishedYes

Keywords

  • Antigen presentation
  • Class switch recombination
  • Heat shock protein 90
  • Humoral immune response
  • IG gene hypermutation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Li, Y., Li, S., Hoshino, M., Ishikawa, R., Kajiwara, C., Gao, X., Zhao, Y., Ishido, S., Udono, H., & Wang, J. Y. (2012). HSP90α deficiency does not affect immunoglobulin gene hypermutation and class switch but causes enhanced MHC class II antigen presentation. International Immunology, 24(12), 751-758. [dxs076]. https://doi.org/10.1093/intimm/dxs076