HSP60 possesses a GTPase activity and mediates protein folding with HSP10

Tomoya Okamoto, Hiroshi Yamamoto, Ikuru Kudo, Kazuya Matsumoto, Masafumi Odaka, Ewa Grave, Hideaki Itoh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The mammalian molecular chaperone, HSP60, plays an essential role in protein homeostasis through mediating protein folding and assembly. The structure and ATP-dependent function of HSP60 has been well established in recent studies. After ATP, GTP is the major cellular nucleotide. In this paper, we have investigated the role of GTP in the activity of HSP60. It was found that HSP60 has different properties with respect to allostery, complex formation and protein folding activity depending on the nucleoside triphosphate present. The presence of GTP slightly affected the ATPase activity of HSP60 during protein folding. These results provide clues as to the functional mechanism of the HSP60-HSP10 complex.

Original languageEnglish
Article number16931
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017
Externally publishedYes

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GTP Phosphohydrolases
Protein Folding
Guanosine Triphosphate
Adenosine Triphosphate
Molecular Chaperones
Nucleosides
Adenosine Triphosphatases
Homeostasis
Nucleotides
Proteins

ASJC Scopus subject areas

  • General

Cite this

Okamoto, T., Yamamoto, H., Kudo, I., Matsumoto, K., Odaka, M., Grave, E., & Itoh, H. (2017). HSP60 possesses a GTPase activity and mediates protein folding with HSP10. Scientific Reports, 7(1), [16931]. https://doi.org/10.1038/s41598-017-17167-7

HSP60 possesses a GTPase activity and mediates protein folding with HSP10. / Okamoto, Tomoya; Yamamoto, Hiroshi; Kudo, Ikuru; Matsumoto, Kazuya; Odaka, Masafumi; Grave, Ewa; Itoh, Hideaki.

In: Scientific Reports, Vol. 7, No. 1, 16931, 01.12.2017.

Research output: Contribution to journalArticle

Okamoto, T, Yamamoto, H, Kudo, I, Matsumoto, K, Odaka, M, Grave, E & Itoh, H 2017, 'HSP60 possesses a GTPase activity and mediates protein folding with HSP10', Scientific Reports, vol. 7, no. 1, 16931. https://doi.org/10.1038/s41598-017-17167-7
Okamoto, Tomoya ; Yamamoto, Hiroshi ; Kudo, Ikuru ; Matsumoto, Kazuya ; Odaka, Masafumi ; Grave, Ewa ; Itoh, Hideaki. / HSP60 possesses a GTPase activity and mediates protein folding with HSP10. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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