HSP-enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells

Kisho Ono, Takanori Eguchi, Chiharu Sogawa, Stuart K. Calderwood, Junya Futagawa, Tomonari Kasai, Masaharu Seno, Kuniaki Okamoto, Akira Sasaki, Ken ichi Kozaki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cancer cells often secrete extracellular vesicles (EVs) that carry heat shock proteins (HSPs) with roles in tumor progression. Oral squamous cell carcinoma (OSCC) belongs to head and neck cancers (HNC) whose lymph-node-metastases often lead to poor prognosis. We have examined the EV proteome of OSCC cells and found abundant secretion of HSP90-enriched EVs in lymph-node-metastatic OSCC cells. Double knockdown of HSP90α and HSP90β, using small interfering RNA significantly reduced the survival of the metastatic OSCC cells, although single knockdown of each HSP90 was ineffective. Elevated expression of these HSP90 family members was found to correlate with poor prognosis of HNC cases. Thus, elevated HSP90 levels in secreted vesicles are potential prognostic biomarkers and therapeutic targets in metastatic OSCC.

Original languageEnglish
Pages (from-to)7350-7362
Number of pages13
JournalJournal of Cellular Biochemistry
Volume119
Issue number9
DOIs
Publication statusPublished - Sep 2018

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Keywords

  • EV proteomics
  • exosome
  • extracellular vesicle
  • heat shock proteins
  • lymph-node-metastatic oral cancer
  • molecular chaperone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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