@article{d59810d7d35b435aa9ba134ab2d6d024,
title = "HSP-enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells",
abstract = "Cancer cells often secrete extracellular vesicles (EVs) that carry heat shock proteins (HSPs) with roles in tumor progression. Oral squamous cell carcinoma (OSCC) belongs to head and neck cancers (HNC) whose lymph-node-metastases often lead to poor prognosis. We have examined the EV proteome of OSCC cells and found abundant secretion of HSP90-enriched EVs in lymph-node-metastatic OSCC cells. Double knockdown of HSP90α and HSP90β, using small interfering RNA significantly reduced the survival of the metastatic OSCC cells, although single knockdown of each HSP90 was ineffective. Elevated expression of these HSP90 family members was found to correlate with poor prognosis of HNC cases. Thus, elevated HSP90 levels in secreted vesicles are potential prognostic biomarkers and therapeutic targets in metastatic OSCC.",
keywords = "EV proteomics, exosome, extracellular vesicle, heat shock proteins, lymph-node-metastatic oral cancer, molecular chaperone",
author = "Kisho Ono and Takanori Eguchi and Chiharu Sogawa and Calderwood, {Stuart K.} and Junya Futagawa and Tomonari Kasai and Masaharu Seno and Kuniaki Okamoto and Akira Sasaki and Ken-ichi Kozaｋi",
note = "Funding Information: This paper is dedicated to the memory of one of the coauthors, Professor Ken-ichi Kozaki, who passed away on May 29, 2016. The authors thank Seiji Tamaru, Haruo Urata, and Kazuko Kobayashi for helpful operation of LC-MS/MS, TEM, and Zetasizer, respectively, and members of department of Dental Pharmacology and of department of Oral and Craniofacial Surgery at Okayama University for support. This work was supported by JSPS KAKENHI, grant numbers JP16K11722 (to JM and TE), JP17K11642 (to TE), and JP17K11669 (to KO, CS, and TE) and a Ryobi Teien Memorial Foundation Grant (to TE). Mitsui Knowledge Industry Co., Ltd. provided support in the form of salaries for JF, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",
year = "2018",
month = sep,
doi = "10.1002/jcb.27039",
language = "English",
volume = "119",
pages = "7350--7362",
journal = "Journal of supramolecular structure and cellular biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "9",
}
