How capping protein binds the barbed end of the actin filament

Martin A. Wear, Atsuko Yamashita, Kyoungtae Kim, Yuichiro Maéda, John A. Cooper

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Cytoskeletal filaments are often capped at one end, regulating assembly and cellular location. The actin filament is a right-handed, two-strand long-pitch helix [1]. The ends of the two protofilaments are staggered in relation to each other, suggesting that capping could result from one protein binding simultaneously to the ends of both protofilaments. Capping protein (CP), a ubiquitous α/β heterodimer in eukaryotes, tightly caps (K d ∼0.1-1 nM) the barbed end of the actin filament (the end favored for polymerization), preventing actin subunit addition and loss [2]. CP is critical for actin assembly and actin-based motility in vivo [2] and is an essential component of the dendritic nucleation model for actin polymerization at the leading edge of cells [3]. However, the mechanism by which CP caps actin filaments is not well understood. The X-ray crystal structure of CP has inspired a model where the C termini (∼30 amino acids) of the α and β subunits of CP are mobile extensions ("tentacles"), and these regions are responsible for high-affinity binding to, and functional capping of, the barbed end [4]. We tested the tentacle model in vitro with recombinant mutant CPs. Loss of both tentacles causes a complete loss of capping activity. The α tentacle contributes more to capping affinity and kinetics; its removal reduces capping affinity by 5000-fold and the on-rate of capping by 20-fold. In contrast, removal of the β tentacle reduced the affinity by only 300-fold and did not affect the on-rate. These two regions are not close to each other in the three-dimensional structure, suggesting CP uses two independent actin binding tentacles to cap the barbed end. CP with either tentacle alone can cap, as can the isolated β tentacle alone, suggesting that the individual tentacles interact with more than one actin subunit at a subunit interface at the barbed end.

Original languageEnglish
Pages (from-to)1531-1537
Number of pages7
JournalCurrent Biology
Volume13
Issue number17
DOIs
Publication statusPublished - Sep 2 2003
Externally publishedYes

Fingerprint

microfilaments
Actin Cytoskeleton
actin
Actins
Proteins
proteins
Polymerization
polymerization
Actin Capping Proteins
protein binding
Protein Subunits
protein subunits
crystal structure
cytoskeleton
Eukaryota
Cytoskeleton
Protein Binding
eukaryotic cells
X-radiation
X-Rays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

How capping protein binds the barbed end of the actin filament. / Wear, Martin A.; Yamashita, Atsuko; Kim, Kyoungtae; Maéda, Yuichiro; Cooper, John A.

In: Current Biology, Vol. 13, No. 17, 02.09.2003, p. 1531-1537.

Research output: Contribution to journalArticle

Wear, Martin A. ; Yamashita, Atsuko ; Kim, Kyoungtae ; Maéda, Yuichiro ; Cooper, John A. / How capping protein binds the barbed end of the actin filament. In: Current Biology. 2003 ; Vol. 13, No. 17. pp. 1531-1537.
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