Host range of Frankia strains isolated from actinorhizal plants growing in Japan and their relatedness based on 16S rDNA

Yuki Nagashima, Chiharu Tani, Mikihiro Yamamoto, Hideo Sasakawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The host range of seven Frankia strains isolated from the root nodules of five actinorhizal plants in four genera growing in Japan and their relatedness based on 16S rDNA were examined. The Ceq1 strain isolated from Casuarina equisetifolia formed many nodules with high nitrogen fixation activity on the source host, C. equisetifolia. The Ceq1 strain formed nodules on Alnus sieboldiana, Alnus hirsta and Myrica rubra, but their nodulation ratio and nitrogen fixation activity were very low. The Asi1 strain isolated from A. sieboldiana, the Ahi1 strain isolated from A. hirsta, and the Mru1, Mru2 and Mru8 strains isolated from M. rubra formed many nodules on both Alnus species and M. rubra. The Ema2 strain isolated from Elaeagnus macrophylla formed nodules on both Elaeagnus species and M. rubra. Based on the aspect of nodulation and nitrogen fixation activity, Ceq1 strain is an effective strain only for Casuarina, five strains of Asi1, Ahi1, Mru1, Mru2 and Mru8 are effective strains for both Alnus and Myrica, and Ema2 strain is an effective strain for both Elaeagnus and Myrica. The results of phylogenetic analyses based on 16S rDNA sequences showed that seven strains were separated into two major clusters. Cluster 1 was further grouped into two subclusters, 1a and 1b. The Asi1, Ahi1, Mru1, Mru2 and Mru8 strains belonged to 1a and the Ceq1 strain belonged to cluster 1b. The Ema2 strain was classified into cluster 2. This phylogenetic classification of Frankia based on 16S rDNA sequences appears to be consistent with the host range determined using an inoculation test.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalSoil Science and Plant Nutrition
Volume54
Issue number3
DOIs
Publication statusPublished - Jun 2008

Fingerprint

actinorhiza
Frankia
host range
nitrogen fixation
relatedness
nodulation
Japan
phylogenetics
Morella rubra
inoculation
Elaeagnus
Myrica
Casuarina equisetifolia
Alnus
actinorhizae

Keywords

  • 16S rDNA
  • Actinorhizal plants
  • Frankia
  • Host range
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Soil Science
  • Agronomy and Crop Science

Cite this

Host range of Frankia strains isolated from actinorhizal plants growing in Japan and their relatedness based on 16S rDNA. / Nagashima, Yuki; Tani, Chiharu; Yamamoto, Mikihiro; Sasakawa, Hideo.

In: Soil Science and Plant Nutrition, Vol. 54, No. 3, 06.2008, p. 379-386.

Research output: Contribution to journalArticle

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abstract = "The host range of seven Frankia strains isolated from the root nodules of five actinorhizal plants in four genera growing in Japan and their relatedness based on 16S rDNA were examined. The Ceq1 strain isolated from Casuarina equisetifolia formed many nodules with high nitrogen fixation activity on the source host, C. equisetifolia. The Ceq1 strain formed nodules on Alnus sieboldiana, Alnus hirsta and Myrica rubra, but their nodulation ratio and nitrogen fixation activity were very low. The Asi1 strain isolated from A. sieboldiana, the Ahi1 strain isolated from A. hirsta, and the Mru1, Mru2 and Mru8 strains isolated from M. rubra formed many nodules on both Alnus species and M. rubra. The Ema2 strain isolated from Elaeagnus macrophylla formed nodules on both Elaeagnus species and M. rubra. Based on the aspect of nodulation and nitrogen fixation activity, Ceq1 strain is an effective strain only for Casuarina, five strains of Asi1, Ahi1, Mru1, Mru2 and Mru8 are effective strains for both Alnus and Myrica, and Ema2 strain is an effective strain for both Elaeagnus and Myrica. The results of phylogenetic analyses based on 16S rDNA sequences showed that seven strains were separated into two major clusters. Cluster 1 was further grouped into two subclusters, 1a and 1b. The Asi1, Ahi1, Mru1, Mru2 and Mru8 strains belonged to 1a and the Ceq1 strain belonged to cluster 1b. The Ema2 strain was classified into cluster 2. This phylogenetic classification of Frankia based on 16S rDNA sequences appears to be consistent with the host range determined using an inoculation test.",
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