Honeydew-associated microbes elicit defense responses against brown planthopper in rice

David Wari, Md Alamgir Kabir, Kadis Mujiono, Yuko Hojo, Tomonori Shinya, Akio Tani, Hiroko Nakatani, Ivan Galis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Feeding of sucking insects, such as the rice brown planthopper (Nilaparvata lugens; BPH), causes only limited mechanical damage on plants that is otherwise essential for injury-triggered defense responses against herbivores. In pursuit of complementary BPH elicitors perceived by plants, we examined the potential effects of BPH honeydew secretions on the BPH monocot host, rice (Oryza sativa). We found that BPH honeydew strongly elicits direct and putative indirect defenses in rice, namely accumulation of phytoalexins in the leaves, and release of volatile organic compounds from the leaves that serve to attract natural enemies of herbivores, respectively. We then examined the elicitor active components in the honeydew and found that bacteria in the secretions are responsible for the activation of plant defense. Corroborating the importance of honeydew-associated microbiota for induced plant resistance, BPHs partially devoid of their microbiota via prolonged antibiotics ingestion induced significantly less defense in rice relative to antibiotic-free insects applied to similar groups of plants. Our data suggest that rice plants may additionally perceive herbivores via their honeydew-associated microbes, allowing them to discriminate between incompatible herbivores-that do not produce honeydew-and those that are compatible and therefore dangerous.

Original languageEnglish
Pages (from-to)1683-1696
Number of pages14
JournalJournal of experimental botany
Volume70
Issue number5
DOIs
Publication statusPublished - Mar 11 2019

Fingerprint

Nilaparvata lugens
honeydew
Herbivory
microorganisms
rice
herbivores
Microbiota
Insects
antibiotics
secretion
Anti-Bacterial Agents
Volatile Organic Compounds
insects
mechanical damage
volatile organic compounds
phytoalexins
Liliopsida
natural enemies
Oryza
leaves

Keywords

  • Honeydew-associated microorganisms
  • phytoalexins
  • plant defense
  • rice (Oryza sativa)
  • rice brown planthopper (Nilaparvata lugens)
  • sucking insect

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Honeydew-associated microbes elicit defense responses against brown planthopper in rice. / Wari, David; Kabir, Md Alamgir; Mujiono, Kadis; Hojo, Yuko; Shinya, Tomonori; Tani, Akio; Nakatani, Hiroko; Galis, Ivan.

In: Journal of experimental botany, Vol. 70, No. 5, 11.03.2019, p. 1683-1696.

Research output: Contribution to journalArticle

Wari, David ; Kabir, Md Alamgir ; Mujiono, Kadis ; Hojo, Yuko ; Shinya, Tomonori ; Tani, Akio ; Nakatani, Hiroko ; Galis, Ivan. / Honeydew-associated microbes elicit defense responses against brown planthopper in rice. In: Journal of experimental botany. 2019 ; Vol. 70, No. 5. pp. 1683-1696.
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