Abstract
Rice is an important staple food for more than half the world’s population, and it has become the first crop plant to have its 389-Mb genome sequenced (IRGSP 2005). Even though various functional genomic tools for elucidating the function of rice genes are available (Hirochika et al. 2004; Leung and An 2004; Sasaki et al. 2005; Upadhyaya 2007), developing new methods for characterizing genes of interest by both forward and reverse genetic approaches has become particularly important. This chapter contains a description of the current state of gene targeting mediated by homologous recombination (HR) and gene tagging promoted by a non-autonomous DNA-based active rice transposon, nDart, for rice functional genomics, both of which are being developed (Terada et al. 2002, 2007; Tsugane et al. 2006; Takagi et al. 2007). Gene targeting refers to the alteration of a specific DNA sequence in an endogenous gene at its original locus in the genome and, often, to the conversion of the endogenous gene into a designed sequence. Gene targeting has been shown to lead to both gene replacements and base changes (Reiss 2003; Iida and Terada 2004, 2005); by the end of 2006, there was only one report describing reproducible true gene targeting (TGT) in rice, and it dealt with the inactivation or knockout of the endogenous Waxy gene by gene disruption (Figs. 1a and 2; Terada et al. 2002). Because independent gene targeting events by HR should generate an identical genomic structure with a designed sequence alteration(s), the experimental demonstration of the capability for the reproducible isolation of recombinants with the anticipated gene structure would be very important.
| Original language | English |
|---|---|
| Title of host publication | Biotechnology in Agriculture and Forestry |
| Publisher | Springer International Publishing |
| Pages | 81-94 |
| Number of pages | 14 |
| Volume | 62 |
| DOIs | |
| Publication status | Published - 2008 |
Publication series
| Name | Biotechnology in Agriculture and Forestry |
|---|---|
| Volume | 62 |
| ISSN (Print) | 0934-943X |
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ASJC Scopus subject areas
- Biotechnology
- Forestry
- Agronomy and Crop Science
Cite this
Homologous recombination-dependent gene targeting and an active DNA transposon nDart-promoted gene tagging for rice functional genomics. / Johzuka-Hisatomi, Yasuyo; Maekawa, Masahiko; Takagi, Kyoko; Eun, Chang Ho; Yamauchi, Takaki; Shimatani, Zenpei; Ahmed, Nisar; Urawa, Hiroko; Tsugane, Kazuo; Terada, Rie; Iida, Shigeru.
Biotechnology in Agriculture and Forestry. Vol. 62 Springer International Publishing, 2008. p. 81-94 (Biotechnology in Agriculture and Forestry; Vol. 62).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Homologous recombination-dependent gene targeting and an active DNA transposon nDart-promoted gene tagging for rice functional genomics
AU - Johzuka-Hisatomi, Yasuyo
AU - Maekawa, Masahiko
AU - Takagi, Kyoko
AU - Eun, Chang Ho
AU - Yamauchi, Takaki
AU - Shimatani, Zenpei
AU - Ahmed, Nisar
AU - Urawa, Hiroko
AU - Tsugane, Kazuo
AU - Terada, Rie
AU - Iida, Shigeru
PY - 2008
Y1 - 2008
N2 - Rice is an important staple food for more than half the world’s population, and it has become the first crop plant to have its 389-Mb genome sequenced (IRGSP 2005). Even though various functional genomic tools for elucidating the function of rice genes are available (Hirochika et al. 2004; Leung and An 2004; Sasaki et al. 2005; Upadhyaya 2007), developing new methods for characterizing genes of interest by both forward and reverse genetic approaches has become particularly important. This chapter contains a description of the current state of gene targeting mediated by homologous recombination (HR) and gene tagging promoted by a non-autonomous DNA-based active rice transposon, nDart, for rice functional genomics, both of which are being developed (Terada et al. 2002, 2007; Tsugane et al. 2006; Takagi et al. 2007). Gene targeting refers to the alteration of a specific DNA sequence in an endogenous gene at its original locus in the genome and, often, to the conversion of the endogenous gene into a designed sequence. Gene targeting has been shown to lead to both gene replacements and base changes (Reiss 2003; Iida and Terada 2004, 2005); by the end of 2006, there was only one report describing reproducible true gene targeting (TGT) in rice, and it dealt with the inactivation or knockout of the endogenous Waxy gene by gene disruption (Figs. 1a and 2; Terada et al. 2002). Because independent gene targeting events by HR should generate an identical genomic structure with a designed sequence alteration(s), the experimental demonstration of the capability for the reproducible isolation of recombinants with the anticipated gene structure would be very important.
AB - Rice is an important staple food for more than half the world’s population, and it has become the first crop plant to have its 389-Mb genome sequenced (IRGSP 2005). Even though various functional genomic tools for elucidating the function of rice genes are available (Hirochika et al. 2004; Leung and An 2004; Sasaki et al. 2005; Upadhyaya 2007), developing new methods for characterizing genes of interest by both forward and reverse genetic approaches has become particularly important. This chapter contains a description of the current state of gene targeting mediated by homologous recombination (HR) and gene tagging promoted by a non-autonomous DNA-based active rice transposon, nDart, for rice functional genomics, both of which are being developed (Terada et al. 2002, 2007; Tsugane et al. 2006; Takagi et al. 2007). Gene targeting refers to the alteration of a specific DNA sequence in an endogenous gene at its original locus in the genome and, often, to the conversion of the endogenous gene into a designed sequence. Gene targeting has been shown to lead to both gene replacements and base changes (Reiss 2003; Iida and Terada 2004, 2005); by the end of 2006, there was only one report describing reproducible true gene targeting (TGT) in rice, and it dealt with the inactivation or knockout of the endogenous Waxy gene by gene disruption (Figs. 1a and 2; Terada et al. 2002). Because independent gene targeting events by HR should generate an identical genomic structure with a designed sequence alteration(s), the experimental demonstration of the capability for the reproducible isolation of recombinants with the anticipated gene structure would be very important.
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U2 - 10.1007/978-3-540-74250-0_7
DO - 10.1007/978-3-540-74250-0_7
M3 - Chapter
VL - 62
T3 - Biotechnology in Agriculture and Forestry
SP - 81
EP - 94
BT - Biotechnology in Agriculture and Forestry
PB - Springer International Publishing
ER -