Abstract
Homo-poly{ N'-[ N-(2-aminoethyl)-2-aminoehtyl]aspartamide} [PAsp(DET), H] was attempted to integrate into poly (ethylene glycol) (PEG)- b-PAsp(DET)] ( B) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration In vitro evaluations verified H integration of potent stimulation in enhancing cell-transfecting activity of PEGylated polyplex micelles via promoted cellular uptake and facilitated endosome escape In vivo anti-angiogenic tumor suppression evaluations validated the feasibility of H integration in promoting gene transfection to the affected cells via systemic administration, where loaded anti-angiogenic gene remarkably expressed in the tumor site, thereby imparting significant inhibitory effect on the growth of vascular endothelial cells, ultimately leading to potent tumor growth suppression These results demonstrated potency of H integration for enhanced transfection activity and potential usage in systemic applications, which could have important implications on the strategic use of H integration in the non-viral gene carrier design.
| Original language | English |
|---|---|
| Pages (from-to) | 4722-4730 |
| Number of pages | 9 |
| Journal | Biomaterials |
| Volume | 33 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - Jun 2012 |
| Externally published | Yes |
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Keywords
- DNA
- Gene transfer
- In vitro test
- In vivo test
- Micelle
- Nanoparticle
ASJC Scopus subject areas
- Biomaterials
- Bioengineering
- Ceramics and Composites
- Mechanics of Materials
- Biophysics
Cite this
Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors. / Chen, Qixian; Osada, Kensuke; Ishii, Takehiko; Oba, Makoto; Uchida, Satoshi; Tockary, Theofilus A.; Endo, Taisuke; Ge, Zhishen; Kinoh, Hiroaki; Kano, Mitsunobu; Itaka, Keiji; Kataoka, Kazunori.
In: Biomaterials, Vol. 33, No. 18, 06.2012, p. 4722-4730.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors
AU - Chen, Qixian
AU - Osada, Kensuke
AU - Ishii, Takehiko
AU - Oba, Makoto
AU - Uchida, Satoshi
AU - Tockary, Theofilus A.
AU - Endo, Taisuke
AU - Ge, Zhishen
AU - Kinoh, Hiroaki
AU - Kano, Mitsunobu
AU - Itaka, Keiji
AU - Kataoka, Kazunori
PY - 2012/6
Y1 - 2012/6
N2 - Homo-poly{ N'-[ N-(2-aminoethyl)-2-aminoehtyl]aspartamide} [PAsp(DET), H] was attempted to integrate into poly (ethylene glycol) (PEG)- b-PAsp(DET)] ( B) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration In vitro evaluations verified H integration of potent stimulation in enhancing cell-transfecting activity of PEGylated polyplex micelles via promoted cellular uptake and facilitated endosome escape In vivo anti-angiogenic tumor suppression evaluations validated the feasibility of H integration in promoting gene transfection to the affected cells via systemic administration, where loaded anti-angiogenic gene remarkably expressed in the tumor site, thereby imparting significant inhibitory effect on the growth of vascular endothelial cells, ultimately leading to potent tumor growth suppression These results demonstrated potency of H integration for enhanced transfection activity and potential usage in systemic applications, which could have important implications on the strategic use of H integration in the non-viral gene carrier design.
AB - Homo-poly{ N'-[ N-(2-aminoethyl)-2-aminoehtyl]aspartamide} [PAsp(DET), H] was attempted to integrate into poly (ethylene glycol) (PEG)- b-PAsp(DET)] ( B) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration In vitro evaluations verified H integration of potent stimulation in enhancing cell-transfecting activity of PEGylated polyplex micelles via promoted cellular uptake and facilitated endosome escape In vivo anti-angiogenic tumor suppression evaluations validated the feasibility of H integration in promoting gene transfection to the affected cells via systemic administration, where loaded anti-angiogenic gene remarkably expressed in the tumor site, thereby imparting significant inhibitory effect on the growth of vascular endothelial cells, ultimately leading to potent tumor growth suppression These results demonstrated potency of H integration for enhanced transfection activity and potential usage in systemic applications, which could have important implications on the strategic use of H integration in the non-viral gene carrier design.
KW - DNA
KW - Gene transfer
KW - In vitro test
KW - In vivo test
KW - Micelle
KW - Nanoparticle
UR - http://www.scopus.com/inward/record.url?scp=84859835238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859835238&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2012.03.017
DO - 10.1016/j.biomaterials.2012.03.017
M3 - Article
C2 - 22444644
AN - SCOPUS:84859835238
VL - 33
SP - 4722
EP - 4730
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 18
ER -