Homeobox family Hoxc localization during murine palate formation

Azumi Hirata, Kentaro Katayama, Takehito Tsuji, Hideto Imura, Nagato Natsume, Toshio Sugahara, Tetsuo Kunieda, Hiroaki Nakamura, Yoshinori Otsuki

Research output: Contribution to journalArticle

Abstract

Homeobox genes play important roles in craniofacial morphogenesis. However, the characteristics of the transcription factor Hoxc during palate formation remain unclear. We examined the immunolocalization patterns of Hoxc5, Hoxc4, and Hoxc6 in palatogenesis of cleft palate (Eh/Eh) mice. On the other hand, mutations in the FGF/FGFR pathway are exclusively associated with syndromic forms of cleft palate. We also examined the immunolocalization of Fgfr1 and Erk1/2 to clarify their relationships with Hoxc in palatogenesis. Some palatal epithelial cells showed Hoxc5 labeling, while almost no labeling of mesenchymal cells was observed in +/+ mice. As palate formation progressed in +/+ mice, Hoxc5, Hoxc4, and Hoxc6 were observed in medial epithelial seam cells. Hoxc5 and Hoxc6 were detected in the oral epithelium. The palatal mesenchyme also showed intense staining for Fgfr1 and Erk1/2 with progression of palate formation. In contrast, the palatal shelves of Eh/Eh mice exhibited impaired horizontal growth and failed to fuse, resulting in a cleft. Hoxc5 was observed in a few epithelial cells and diffusely in the mesenchyme of Eh/Eh palatal shelves. No or little labeling of Fgfr1 and Erk1/2 was detected in the cleft palate of Eh/Eh mice. These findings suggest that Hoxc genes are involved in palatogenesis. Furthermore, there may be the differences in the localization pattern between Hoxc5, Hoxc4, and Hoxc6. Additionally, Hoxc distribution in palatal cells during palate development may be correlated with FGF signaling. (228/250 words)

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalCongenital Anomalies
Volume56
Issue number4
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Palate
Homeobox Genes
Cleft Palate
Epithelial Cells
Mesoderm
Morphogenesis
Transcription Factors
Epithelium
Staining and Labeling
Mutation
Growth
Genes

Keywords

  • cleft palate
  • Eh/Eh mouse
  • Fgf signaling
  • Hoxc
  • palate formation

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Embryology
  • Developmental Biology

Cite this

Hirata, A., Katayama, K., Tsuji, T., Imura, H., Natsume, N., Sugahara, T., ... Otsuki, Y. (2016). Homeobox family Hoxc localization during murine palate formation. Congenital Anomalies, 56(4), 172-179. https://doi.org/10.1111/cga.12153

Homeobox family Hoxc localization during murine palate formation. / Hirata, Azumi; Katayama, Kentaro; Tsuji, Takehito; Imura, Hideto; Natsume, Nagato; Sugahara, Toshio; Kunieda, Tetsuo; Nakamura, Hiroaki; Otsuki, Yoshinori.

In: Congenital Anomalies, Vol. 56, No. 4, 01.07.2016, p. 172-179.

Research output: Contribution to journalArticle

Hirata, A, Katayama, K, Tsuji, T, Imura, H, Natsume, N, Sugahara, T, Kunieda, T, Nakamura, H & Otsuki, Y 2016, 'Homeobox family Hoxc localization during murine palate formation', Congenital Anomalies, vol. 56, no. 4, pp. 172-179. https://doi.org/10.1111/cga.12153
Hirata A, Katayama K, Tsuji T, Imura H, Natsume N, Sugahara T et al. Homeobox family Hoxc localization during murine palate formation. Congenital Anomalies. 2016 Jul 1;56(4):172-179. https://doi.org/10.1111/cga.12153
Hirata, Azumi ; Katayama, Kentaro ; Tsuji, Takehito ; Imura, Hideto ; Natsume, Nagato ; Sugahara, Toshio ; Kunieda, Tetsuo ; Nakamura, Hiroaki ; Otsuki, Yoshinori. / Homeobox family Hoxc localization during murine palate formation. In: Congenital Anomalies. 2016 ; Vol. 56, No. 4. pp. 172-179.
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