Holocentric chromosomes of luzula elegans are characterized by a longitudinal centromere groove, chromosome bending, and a terminal nucleolus organizer region

S. Heckmann, E. Schroeder-Reiter, K. Kumke, L. Ma, Kiyotaka Nagaki, M. Murata, G. Wanner, A. Houben

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid. The centromere-specific histone H3 variant, CENH3, colocalized with this groove and with microtubule attachment sites. The terminal chromosomal regions were CENH3-negative. During metaphase to anaphase transition, L. elegans chromosomes typically curved to a sickle-like shape, a process that is likely to be influenced by the pulling forces of microtubules along the holocentric axis towards the corresponding microtubule organizing regions. A single pair of 45S rDNA sites, situated distal to Arabidopsis-telomere repeats, was observed at the terminal region of one chromosome pair. We suggest that the 45S rDNA position in distal centromere-free regions could be required to ensure chromosome stability.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalCytogenetic and Genome Research
Volume134
Issue number3
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Nucleolus Organizer Region
Centromere
Microtubules
Chromosomes
Ribosomal DNA
Chromosome Structures
Chromosomal Instability
Anaphase
Chromatids
Telomere
Metaphase
Arabidopsis
Electron Scanning Microscopy
Histones
Light

Keywords

  • CENH3
  • Centromere
  • Holocentric chromosome
  • Luzula elegans
  • Mitosis
  • rDNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Holocentric chromosomes of luzula elegans are characterized by a longitudinal centromere groove, chromosome bending, and a terminal nucleolus organizer region. / Heckmann, S.; Schroeder-Reiter, E.; Kumke, K.; Ma, L.; Nagaki, Kiyotaka; Murata, M.; Wanner, G.; Houben, A.

In: Cytogenetic and Genome Research, Vol. 134, No. 3, 06.2011, p. 220-228.

Research output: Contribution to journalArticle

Heckmann, S. ; Schroeder-Reiter, E. ; Kumke, K. ; Ma, L. ; Nagaki, Kiyotaka ; Murata, M. ; Wanner, G. ; Houben, A. / Holocentric chromosomes of luzula elegans are characterized by a longitudinal centromere groove, chromosome bending, and a terminal nucleolus organizer region. In: Cytogenetic and Genome Research. 2011 ; Vol. 134, No. 3. pp. 220-228.
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