Holocentric chromosomes of luzula elegans are characterized by a longitudinal centromere groove, chromosome bending, and a terminal nucleolus organizer region

S. Heckmann, E. Schroeder-Reiter, K. Kumke, L. Ma, K. Nagaki, M. Murata, G. Wanner, A. Houben

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid. The centromere-specific histone H3 variant, CENH3, colocalized with this groove and with microtubule attachment sites. The terminal chromosomal regions were CENH3-negative. During metaphase to anaphase transition, L. elegans chromosomes typically curved to a sickle-like shape, a process that is likely to be influenced by the pulling forces of microtubules along the holocentric axis towards the corresponding microtubule organizing regions. A single pair of 45S rDNA sites, situated distal to Arabidopsis-telomere repeats, was observed at the terminal region of one chromosome pair. We suggest that the 45S rDNA position in distal centromere-free regions could be required to ensure chromosome stability.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalCytogenetic and Genome Research
Volume134
Issue number3
DOIs
Publication statusPublished - Jun 2011

Keywords

  • CENH3
  • Centromere
  • Holocentric chromosome
  • Luzula elegans
  • Mitosis
  • rDNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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