Hoechst 33342 is a useful cell trace for a long-term investigation of articular cartilage repair

Keisuke Kawasaki, Shinsuke Sugihara, Keiichiro Nishida, Toshihumi Ozaki, Aki Yoshida, Aiji Ohtsuka, Hajime Inoue

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The repair process of a full-thickness osteochondral defect was observed in a rat model using Hoechst 33342 as a cell tracer. The osteochondral defect was created at the medial femoral condyle of the right knee joints of twelve 11-week-old male rats. Three weeks after the surgery, Hoechst 33342 was injected into the same knee joints. Calcein, a marker of the mineralization front, was then injected subcutaneousely twice at seven days and one day before harvesting of the tissue. At six, ten, and fourteen weeks and one year after the surgery, femoral condyles were obtained from the operated knee joints, fixed by alcohol, and embedded in polymethylmethacrylate. The sections were examined by fluorescent and then light microscopy. In the lateral femoral condyle cartilage, Hoechst 33342 labeling of chondrocyte nuclei was observed in all layers of the intact cartilage, and the dye never infiltrated beneath the subchondral bone plate. At 6 weeks after the surgery, Hoechst 33342-positive cells were observed not only in the regenerated fibrous cartilage, but also in the newly formed mineralized tissue in the medial femoral condyle. Interestingly, Hoechst 33342 labeling remained undiminished even one year after the intraarticular injection. The findings of the present study suggest that intra-articular injection of Hoechst 33342 is a useful tracer for long-term investigations of chondrocyte differentiation in vivo.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalArchives of Histology and Cytology
Volume67
Issue number1
DOIs
Publication statusPublished - Mar 2004

Fingerprint

Articular Cartilage
Thigh
Knee Joint
Cartilage
Bone and Bones
Intra-Articular Injections
Chondrocytes
Tissue and Organ Harvesting
Bone Plates
Polymethyl Methacrylate
HOE 33342
Microscopy
Coloring Agents
Alcohols
Light

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Hoechst 33342 is a useful cell trace for a long-term investigation of articular cartilage repair. / Kawasaki, Keisuke; Sugihara, Shinsuke; Nishida, Keiichiro; Ozaki, Toshihumi; Yoshida, Aki; Ohtsuka, Aiji; Inoue, Hajime.

In: Archives of Histology and Cytology, Vol. 67, No. 1, 03.2004, p. 13-19.

Research output: Contribution to journalArticle

@article{9a4fb432aff041438a649cc8a3a7dfb3,
title = "Hoechst 33342 is a useful cell trace for a long-term investigation of articular cartilage repair",
abstract = "The repair process of a full-thickness osteochondral defect was observed in a rat model using Hoechst 33342 as a cell tracer. The osteochondral defect was created at the medial femoral condyle of the right knee joints of twelve 11-week-old male rats. Three weeks after the surgery, Hoechst 33342 was injected into the same knee joints. Calcein, a marker of the mineralization front, was then injected subcutaneousely twice at seven days and one day before harvesting of the tissue. At six, ten, and fourteen weeks and one year after the surgery, femoral condyles were obtained from the operated knee joints, fixed by alcohol, and embedded in polymethylmethacrylate. The sections were examined by fluorescent and then light microscopy. In the lateral femoral condyle cartilage, Hoechst 33342 labeling of chondrocyte nuclei was observed in all layers of the intact cartilage, and the dye never infiltrated beneath the subchondral bone plate. At 6 weeks after the surgery, Hoechst 33342-positive cells were observed not only in the regenerated fibrous cartilage, but also in the newly formed mineralized tissue in the medial femoral condyle. Interestingly, Hoechst 33342 labeling remained undiminished even one year after the intraarticular injection. The findings of the present study suggest that intra-articular injection of Hoechst 33342 is a useful tracer for long-term investigations of chondrocyte differentiation in vivo.",
author = "Keisuke Kawasaki and Shinsuke Sugihara and Keiichiro Nishida and Toshihumi Ozaki and Aki Yoshida and Aiji Ohtsuka and Hajime Inoue",
year = "2004",
month = "3",
doi = "10.1679/aohc.67.13",
language = "English",
volume = "67",
pages = "13--19",
journal = "Archives of Histology and Cytology",
issn = "0914-9465",
publisher = "Japan Society of Histological Documentation",
number = "1",

}

TY - JOUR

T1 - Hoechst 33342 is a useful cell trace for a long-term investigation of articular cartilage repair

AU - Kawasaki, Keisuke

AU - Sugihara, Shinsuke

AU - Nishida, Keiichiro

AU - Ozaki, Toshihumi

AU - Yoshida, Aki

AU - Ohtsuka, Aiji

AU - Inoue, Hajime

PY - 2004/3

Y1 - 2004/3

N2 - The repair process of a full-thickness osteochondral defect was observed in a rat model using Hoechst 33342 as a cell tracer. The osteochondral defect was created at the medial femoral condyle of the right knee joints of twelve 11-week-old male rats. Three weeks after the surgery, Hoechst 33342 was injected into the same knee joints. Calcein, a marker of the mineralization front, was then injected subcutaneousely twice at seven days and one day before harvesting of the tissue. At six, ten, and fourteen weeks and one year after the surgery, femoral condyles were obtained from the operated knee joints, fixed by alcohol, and embedded in polymethylmethacrylate. The sections were examined by fluorescent and then light microscopy. In the lateral femoral condyle cartilage, Hoechst 33342 labeling of chondrocyte nuclei was observed in all layers of the intact cartilage, and the dye never infiltrated beneath the subchondral bone plate. At 6 weeks after the surgery, Hoechst 33342-positive cells were observed not only in the regenerated fibrous cartilage, but also in the newly formed mineralized tissue in the medial femoral condyle. Interestingly, Hoechst 33342 labeling remained undiminished even one year after the intraarticular injection. The findings of the present study suggest that intra-articular injection of Hoechst 33342 is a useful tracer for long-term investigations of chondrocyte differentiation in vivo.

AB - The repair process of a full-thickness osteochondral defect was observed in a rat model using Hoechst 33342 as a cell tracer. The osteochondral defect was created at the medial femoral condyle of the right knee joints of twelve 11-week-old male rats. Three weeks after the surgery, Hoechst 33342 was injected into the same knee joints. Calcein, a marker of the mineralization front, was then injected subcutaneousely twice at seven days and one day before harvesting of the tissue. At six, ten, and fourteen weeks and one year after the surgery, femoral condyles were obtained from the operated knee joints, fixed by alcohol, and embedded in polymethylmethacrylate. The sections were examined by fluorescent and then light microscopy. In the lateral femoral condyle cartilage, Hoechst 33342 labeling of chondrocyte nuclei was observed in all layers of the intact cartilage, and the dye never infiltrated beneath the subchondral bone plate. At 6 weeks after the surgery, Hoechst 33342-positive cells were observed not only in the regenerated fibrous cartilage, but also in the newly formed mineralized tissue in the medial femoral condyle. Interestingly, Hoechst 33342 labeling remained undiminished even one year after the intraarticular injection. The findings of the present study suggest that intra-articular injection of Hoechst 33342 is a useful tracer for long-term investigations of chondrocyte differentiation in vivo.

UR - http://www.scopus.com/inward/record.url?scp=1942485915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1942485915&partnerID=8YFLogxK

U2 - 10.1679/aohc.67.13

DO - 10.1679/aohc.67.13

M3 - Article

C2 - 15125019

AN - SCOPUS:1942485915

VL - 67

SP - 13

EP - 19

JO - Archives of Histology and Cytology

JF - Archives of Histology and Cytology

SN - 0914-9465

IS - 1

ER -