HMG Domain containing SSRP1 is required for DNA demethylation and genomic imprinting in arabidopsis

Yoko Ikeda, Yuki Kinoshita, Daichi Susaki, Yuriko Ikeda, Megumi Iwano, Seiji Takayama, Tetsuya Higashiyama, Tetsuji Kakutani, Tetsu Kinoshita

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

In Arabidopsis, DEMETER (DME) DNA demethylase contributes to reprogramming of the epigenetic state of the genome in the central cell. However, other aspects of the active DNA demethylation processes remain elusive. Here we show that Arabidopsis SSRP1, known as an HMG domain-containing component of FACT histone chaperone, is required for DNA demethylation and for activation and repression of many parentally imprinted genes in the central cell. Although loss of DNA methylation releases silencing of the imprinted FWA-GFP, double ssrp1-3;. met1-3 mutants surprisingly showed limited activation of maternal FWA-GFP in the central cell, and only became fully active after several nuclear divisions in the endosperm. This behavior was in contrast to the dme-1;. met1 double mutant in which hypomethylation of FWA-GFP by met1 suppressed the DNA demethylation defect of dme-1. We propose that active DNA demethylation by DME requires SSRP1 function through a distinctly different process from direct DNA methylation control.

Original languageEnglish
Pages (from-to)589-596
Number of pages8
JournalDevelopmental Cell
Volume21
Issue number3
DOIs
Publication statusPublished - Sep 13 2011
Externally publishedYes

Fingerprint

Genomic Imprinting
Arabidopsis
DNA
DNA Methylation
Histone Chaperones
Genes
Chemical activation
Cell Nucleus Division
Endosperm
Epigenomics
Mothers
Genome
Defects

ASJC Scopus subject areas

  • Developmental Biology

Cite this

HMG Domain containing SSRP1 is required for DNA demethylation and genomic imprinting in arabidopsis. / Ikeda, Yoko; Kinoshita, Yuki; Susaki, Daichi; Ikeda, Yuriko; Iwano, Megumi; Takayama, Seiji; Higashiyama, Tetsuya; Kakutani, Tetsuji; Kinoshita, Tetsu.

In: Developmental Cell, Vol. 21, No. 3, 13.09.2011, p. 589-596.

Research output: Contribution to journalArticle

Ikeda, Y, Kinoshita, Y, Susaki, D, Ikeda, Y, Iwano, M, Takayama, S, Higashiyama, T, Kakutani, T & Kinoshita, T 2011, 'HMG Domain containing SSRP1 is required for DNA demethylation and genomic imprinting in arabidopsis', Developmental Cell, vol. 21, no. 3, pp. 589-596. https://doi.org/10.1016/j.devcel.2011.08.013
Ikeda, Yoko ; Kinoshita, Yuki ; Susaki, Daichi ; Ikeda, Yuriko ; Iwano, Megumi ; Takayama, Seiji ; Higashiyama, Tetsuya ; Kakutani, Tetsuji ; Kinoshita, Tetsu. / HMG Domain containing SSRP1 is required for DNA demethylation and genomic imprinting in arabidopsis. In: Developmental Cell. 2011 ; Vol. 21, No. 3. pp. 589-596.
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