Histopathological changes induced by zinc hydroxide in rat lungs

H. Ishiyama, Keiki Ogino, M. Sato, M. Ogura, S. Dan, T. Hobara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalExperimental and Toxicologic Pathology
Volume49
Issue number3-4
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Rats
Alveolar Macrophages
Lung
Formazans
Zinc Sulfate
Bronchioles
Proliferating Cell Nuclear Antigen
Oxides
Labeling
Anions
zinc hydroxide
Collagen
Deposits
Staining and Labeling
Injections
Fibers
Therapeutics

Keywords

  • Alveolar macrophages
  • Fibrosis
  • Lung
  • Nitro blue tetrazolium
  • Proliferating cell nuclear antigen
  • Zinc hydroxide
  • Zinc sulfate

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Ishiyama, H., Ogino, K., Sato, M., Ogura, M., Dan, S., & Hobara, T. (1997). Histopathological changes induced by zinc hydroxide in rat lungs. Experimental and Toxicologic Pathology, 49(3-4), 261-266.

Histopathological changes induced by zinc hydroxide in rat lungs. / Ishiyama, H.; Ogino, Keiki; Sato, M.; Ogura, M.; Dan, S.; Hobara, T.

In: Experimental and Toxicologic Pathology, Vol. 49, No. 3-4, 1997, p. 261-266.

Research output: Contribution to journalArticle

Ishiyama, H, Ogino, K, Sato, M, Ogura, M, Dan, S & Hobara, T 1997, 'Histopathological changes induced by zinc hydroxide in rat lungs', Experimental and Toxicologic Pathology, vol. 49, no. 3-4, pp. 261-266.
Ishiyama, H. ; Ogino, Keiki ; Sato, M. ; Ogura, M. ; Dan, S. ; Hobara, T. / Histopathological changes induced by zinc hydroxide in rat lungs. In: Experimental and Toxicologic Pathology. 1997 ; Vol. 49, No. 3-4. pp. 261-266.
@article{a5393de0b1ec4851b4dd818a713383e8,
title = "Histopathological changes induced by zinc hydroxide in rat lungs",
abstract = "Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.",
keywords = "Alveolar macrophages, Fibrosis, Lung, Nitro blue tetrazolium, Proliferating cell nuclear antigen, Zinc hydroxide, Zinc sulfate",
author = "H. Ishiyama and Keiki Ogino and M. Sato and M. Ogura and S. Dan and T. Hobara",
year = "1997",
language = "English",
volume = "49",
pages = "261--266",
journal = "Experimental and Toxicologic Pathology",
issn = "0940-2993",
publisher = "Urban und Fischer Verlag Jena",
number = "3-4",

}

TY - JOUR

T1 - Histopathological changes induced by zinc hydroxide in rat lungs

AU - Ishiyama, H.

AU - Ogino, Keiki

AU - Sato, M.

AU - Ogura, M.

AU - Dan, S.

AU - Hobara, T.

PY - 1997

Y1 - 1997

N2 - Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.

AB - Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.

KW - Alveolar macrophages

KW - Fibrosis

KW - Lung

KW - Nitro blue tetrazolium

KW - Proliferating cell nuclear antigen

KW - Zinc hydroxide

KW - Zinc sulfate

UR - http://www.scopus.com/inward/record.url?scp=0030767760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030767760&partnerID=8YFLogxK

M3 - Article

VL - 49

SP - 261

EP - 266

JO - Experimental and Toxicologic Pathology

JF - Experimental and Toxicologic Pathology

SN - 0940-2993

IS - 3-4

ER -