Abstract
Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.
| Original language | English |
|---|---|
| Pages (from-to) | 261-266 |
| Number of pages | 6 |
| Journal | Experimental and Toxicologic Pathology |
| Volume | 49 |
| Issue number | 3-4 |
| Publication status | Published - 1997 |
| Externally published | Yes |
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Keywords
- Alveolar macrophages
- Fibrosis
- Lung
- Nitro blue tetrazolium
- Proliferating cell nuclear antigen
- Zinc hydroxide
- Zinc sulfate
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Histopathological changes induced by zinc hydroxide in rat lungs. / Ishiyama, H.; Ogino, Keiki; Sato, M.; Ogura, M.; Dan, S.; Hobara, T.
In: Experimental and Toxicologic Pathology, Vol. 49, No. 3-4, 1997, p. 261-266.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Histopathological changes induced by zinc hydroxide in rat lungs
AU - Ishiyama, H.
AU - Ogino, Keiki
AU - Sato, M.
AU - Ogura, M.
AU - Dan, S.
AU - Hobara, T.
PY - 1997
Y1 - 1997
N2 - Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.
AB - Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.
KW - Alveolar macrophages
KW - Fibrosis
KW - Lung
KW - Nitro blue tetrazolium
KW - Proliferating cell nuclear antigen
KW - Zinc hydroxide
KW - Zinc sulfate
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UR - http://www.scopus.com/inward/citedby.url?scp=0030767760&partnerID=8YFLogxK
M3 - Article
VL - 49
SP - 261
EP - 266
JO - Experimental and Toxicologic Pathology
JF - Experimental and Toxicologic Pathology
SN - 0940-2993
IS - 3-4
ER -