Histopathological changes induced by zinc hydroxide in rat lungs

H. Ishiyama, K. Ogino, M. Sato, M. Ogura, S. Dan, T. Hobara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalExperimental and Toxicologic Pathology
Volume49
Issue number3-4
DOIs
Publication statusPublished - Aug 1997

Keywords

  • Alveolar macrophages
  • Fibrosis
  • Lung
  • Nitro blue tetrazolium
  • Proliferating cell nuclear antigen
  • Zinc hydroxide
  • Zinc sulfate

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology

Fingerprint Dive into the research topics of 'Histopathological changes induced by zinc hydroxide in rat lungs'. Together they form a unique fingerprint.

  • Cite this

    Ishiyama, H., Ogino, K., Sato, M., Ogura, M., Dan, S., & Hobara, T. (1997). Histopathological changes induced by zinc hydroxide in rat lungs. Experimental and Toxicologic Pathology, 49(3-4), 261-266. https://doi.org/10.1016/S0940-2993(97)80027-9