TY - JOUR
T1 - Histopathological changes induced by zinc hydroxide in rat lungs
AU - Ishiyama, H.
AU - Ogino, K.
AU - Sato, M.
AU - Ogura, M.
AU - Dan, S.
AU - Hobara, T.
N1 - Funding Information:
Acknowledgement: This work was supported, in part, by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.
PY - 1997/8
Y1 - 1997/8
N2 - Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.
AB - Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with in filtration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.
KW - Alveolar macrophages
KW - Fibrosis
KW - Lung
KW - Nitro blue tetrazolium
KW - Proliferating cell nuclear antigen
KW - Zinc hydroxide
KW - Zinc sulfate
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U2 - 10.1016/S0940-2993(97)80027-9
DO - 10.1016/S0940-2993(97)80027-9
M3 - Article
C2 - 9314062
AN - SCOPUS:0030767760
SN - 0940-2993
VL - 49
SP - 261
EP - 266
JO - Experimentelle Pathologie
JF - Experimentelle Pathologie
IS - 3-4
ER -