Abstract
Bleomycin induces single- and double-stranded breaks in DNA, with consequent mitochondrial membrane aberrations that lead to the apoptotic cell death. It is poorly understood how DNA damage-inducing apoptotic signals are transmitted to mitochondria, from which apoptotic factors are released into the cytoplasm. Here, we investigated the localization of histone H1.2 in the bleomycin-treated human squamous carcinoma SCCTF cells. The presence of DNA double-strand breaks in the bleomycin-treated cells was examined by Western analysis using antibody against phosphorylated histone H2AX (γ-H2AX). Incubation of SCCTF cells for 48 h with 10 μM bleomycin induced apoptosis, as determined by cleavage of lamin B1 to 28 kDa fragment and DNA ladder formation. The mitochondrial permeabilization causing apoptotic feature was also detected with MitoCapture in the bleomycin-treated cells. Histone H1.2 was translocated from the nucleus to the mitochondria after treatment with bleomycin and co-localized with Bak in mitochondria. Our present results suggest that histone H1.2 plays an important role in transmitting apoptotic signals from the nucleus to the mitochondria following double-stranded breaks of DNA by bleomycin.
| Original language | English |
|---|---|
| Pages (from-to) | 1488-1496 |
| Number of pages | 9 |
| Journal | Journal of Cellular Biochemistry |
| Volume | 103 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Apr 1 2008 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Bak
- Bleomycin
- H1.2
- Mitochondria
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
Cite this
Histone H1.2 is translocated to mitochondria and associates with bak in bleomycin-induced apoptotic cells. / Okamura, Hirohiko; Yoshida, Kaya; Amorim, Bruna Rabelo; Haneji, Tatsuji.
In: Journal of Cellular Biochemistry, Vol. 103, No. 5, 01.04.2008, p. 1488-1496.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Histone H1.2 is translocated to mitochondria and associates with bak in bleomycin-induced apoptotic cells
AU - Okamura, Hirohiko
AU - Yoshida, Kaya
AU - Amorim, Bruna Rabelo
AU - Haneji, Tatsuji
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Bleomycin induces single- and double-stranded breaks in DNA, with consequent mitochondrial membrane aberrations that lead to the apoptotic cell death. It is poorly understood how DNA damage-inducing apoptotic signals are transmitted to mitochondria, from which apoptotic factors are released into the cytoplasm. Here, we investigated the localization of histone H1.2 in the bleomycin-treated human squamous carcinoma SCCTF cells. The presence of DNA double-strand breaks in the bleomycin-treated cells was examined by Western analysis using antibody against phosphorylated histone H2AX (γ-H2AX). Incubation of SCCTF cells for 48 h with 10 μM bleomycin induced apoptosis, as determined by cleavage of lamin B1 to 28 kDa fragment and DNA ladder formation. The mitochondrial permeabilization causing apoptotic feature was also detected with MitoCapture in the bleomycin-treated cells. Histone H1.2 was translocated from the nucleus to the mitochondria after treatment with bleomycin and co-localized with Bak in mitochondria. Our present results suggest that histone H1.2 plays an important role in transmitting apoptotic signals from the nucleus to the mitochondria following double-stranded breaks of DNA by bleomycin.
AB - Bleomycin induces single- and double-stranded breaks in DNA, with consequent mitochondrial membrane aberrations that lead to the apoptotic cell death. It is poorly understood how DNA damage-inducing apoptotic signals are transmitted to mitochondria, from which apoptotic factors are released into the cytoplasm. Here, we investigated the localization of histone H1.2 in the bleomycin-treated human squamous carcinoma SCCTF cells. The presence of DNA double-strand breaks in the bleomycin-treated cells was examined by Western analysis using antibody against phosphorylated histone H2AX (γ-H2AX). Incubation of SCCTF cells for 48 h with 10 μM bleomycin induced apoptosis, as determined by cleavage of lamin B1 to 28 kDa fragment and DNA ladder formation. The mitochondrial permeabilization causing apoptotic feature was also detected with MitoCapture in the bleomycin-treated cells. Histone H1.2 was translocated from the nucleus to the mitochondria after treatment with bleomycin and co-localized with Bak in mitochondria. Our present results suggest that histone H1.2 plays an important role in transmitting apoptotic signals from the nucleus to the mitochondria following double-stranded breaks of DNA by bleomycin.
KW - Apoptosis
KW - Bak
KW - Bleomycin
KW - H1.2
KW - Mitochondria
UR - http://www.scopus.com/inward/record.url?scp=41749084159&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41749084159&partnerID=8YFLogxK
U2 - 10.1002/jcb.21537
DO - 10.1002/jcb.21537
M3 - Article
C2 - 17879944
AN - SCOPUS:41749084159
VL - 103
SP - 1488
EP - 1496
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 5
ER -