Histological signs of immune reactions against allogeneic solid fetal neural grafts in the mouse cerebellum depend on the MHC locus

I. Date, K. Kawamura, H. Nakashima

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Using an immunocytochemical method, we examined the immunological responses of adult mice to intracerebellar syngeneic and allogeneic fetal mouse brainstem transplants (embryonic days 12-14). Syngeneic grafts and major histocompatibility complex (MHC)-compatible and non-MHC-incompatible allogeneic grafts survived well, showing no histological signs of rejection even 6 months after transplantation, and with no expression of MHC antigens being observed in any of the grafts. However, most cases of both MHC- and non-MHC-incompatible allografts showed rejection responses, such as marked neovascularization, cellular infiltration and necrosis, two weeks to one month after transplantation. In animals showing rejection, Class I MHC antigens were found on grafted neuronal tissue. An increased number of reactive astrocytes was also observed in the grafts. High levels of Class I antigen expression and prominent gliosis correlated with vigorous cellular infiltration. A quantitative analysis of T cell subsets in the animals showing rejection revealed that the L3T4/Lyt-2 ratio was 1.02±0.21 (mean ± S.D.), indicating that helper/inducer and cytotoxic/suppressor T cells appeared equally in the rejection of MHC- and non-MHC-incompatible allografts. We consider that in these experiments, the brain was not completely an immunologically privileged site, and that MHC- and non-MHC-incompatible intraparenchymal neural transplants were not shielded from host immune surveillance.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalExperimental Brain Research
Volume73
Issue number1
DOIs
Publication statusPublished - Oct 1988

Keywords

  • Allograft
  • Major histocompatibility complex
  • Neural transplantation
  • Rejection
  • T cell subset

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Histological signs of immune reactions against allogeneic solid fetal neural grafts in the mouse cerebellum depend on the MHC locus'. Together they form a unique fingerprint.

  • Cite this