Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

Yasuhisa Kobayashi, Ryo Nozu, Ryo Horiguchi, Masaru Nakamura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Doublesex-mab 3-related transcription factor 1 (DMRT1) has been identified as the first conserved gene involved in the testicular differentiation of vertebrates. However, the precise role of DMRT1 in spermatogenesis has not been made clear. In this study, immunohistochemical method was used to observe DMRT1 protein localization in order to resolve cellular profile of DMRT1 in the adult testis of three-spot wrasse. DMRT1 protein was clearly and specifically localized in the Sertoli cells of all spermatogenic cells and epithelial cells comprising the efferent duct, but not in the germ cells. In addition, adult males were treated with aromatase inhibitor (AI) for investigating the role of estrogen on the transcription of DMRT1. AI treatment caused an increase in the levels of DMRT1 transcripts in the efferent duct region, concomitant with a decrease in spermatogonia and spermatocytes.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalInternational Aquatic Research
Volume6
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Labridae
inhibitor
testes
transcription factors
germ cell
protein
vertebrate
gene
spermatogonia
Sertoli cells
spermatocytes
spermatogenesis
estrogens
germ cells
epithelial cells
proteins
transcription (genetics)
vertebrates
method

Keywords

  • Aromatase inhibitor
  • DMRT1
  • Efferent duct
  • Sertoli cell
  • Spermatogenesis
  • Wrasse

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse. / Kobayashi, Yasuhisa; Nozu, Ryo; Horiguchi, Ryo; Nakamura, Masaru.

In: International Aquatic Research, Vol. 6, No. 2, 2014, p. 1-9.

Research output: Contribution to journalArticle

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