Histological evaluation of periodontal ligament in response to orthodontic mechanical stress in mice

Keiko Kaneko, Saeka Matsuda, Rina Muraoka, Keisuke Nakano, Takami Iwasaki, Mihoko Tomida, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Toshiyuki Kawakami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The purpose of the study was to determine the cell dynamics in periodontal ligament in response to mechanical stress during orthodontic movement. Following Waldo’s method, a square sheet of rubber dam was inserted in between the first and second maxillary molars in 10 ddY mice leaving the stress load for 3 hours. After 3 days and at 1 week, cell count on pressure and tension sides of the periodontal ligament was determined. Furthermore, the type of cell present after mechanical stress was identified using GFP bone marrow transplantation mouse model. Immunohistochemistry was carried out at 0 min (immediately after mechanical stress), 24 hours, 1 week, 2 weeks and 6 months. Temporal changes in the expression of GFP-positive bone marrow derived cells were examined. Moreover, double immunofluorescent staining was performed to determine the type of cell in the periodontal ligament. Cell count on the tension side tremendously increased 3 days after mechanical stress. At 1 week, spindle and round cell count increased compared to the control group. These changes were observed on both tension and pressure sides. Cell count on pressure side at 3 days (22.11+/-13.98) and at 1 week (33.23+/-11.39) was higher compared to the control group (15.26+/-8.29). On the tension side, there was a significantly increased at 3 days (35.46+/-11.85), but decreased at 1 week (29.23+/-13.89) although it is still higher compared to the control group (AD+/-SD: 10.37+/-8.69). Using GFP bone marrow transplantation mouse model, GFP positive cell count increased gradually over time in 6 months. GFP positive cells were also positive to CD31, CD68 and Runx2 suggesting that fibroblasts differentiated into osteoclasts and tissue macrophages. In conclusion, mechanical stress during orthodontic movement promoted the increase in the number of cells in the periodontal ligament on both tension and pressure sides. The increase in the number of cells in the periodontal ligament is believed to be due to the migration and cell division of undifferentiated mesenchymal cells.

Original languageEnglish
Pages (from-to)689-694
Number of pages6
JournalInternational Journal of Medical Sciences
Volume12
Issue number9
DOIs
Publication statusPublished - Aug 14 2015

Fingerprint

Periodontal Ligament
Mechanical Stress
Orthodontics
Cell Count
Pressure
Bone Marrow Transplantation
Control Groups
Rubber Dams
Osteoclasts
Bone Marrow Cells
Cell Division
Fibroblasts
Immunohistochemistry
Macrophages
Staining and Labeling

Keywords

  • Cell differentiation
  • Cell movement
  • Immunohistochemistry
  • Orthodontic mechanical stress
  • Periodontal ligament

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Histological evaluation of periodontal ligament in response to orthodontic mechanical stress in mice. / Kaneko, Keiko; Matsuda, Saeka; Muraoka, Rina; Nakano, Keisuke; Iwasaki, Takami; Tomida, Mihoko; Tsujigiwa, Hidetsugu; Nagatsuka, Hitoshi; Kawakami, Toshiyuki.

In: International Journal of Medical Sciences, Vol. 12, No. 9, 14.08.2015, p. 689-694.

Research output: Contribution to journalArticle

Kaneko, Keiko ; Matsuda, Saeka ; Muraoka, Rina ; Nakano, Keisuke ; Iwasaki, Takami ; Tomida, Mihoko ; Tsujigiwa, Hidetsugu ; Nagatsuka, Hitoshi ; Kawakami, Toshiyuki. / Histological evaluation of periodontal ligament in response to orthodontic mechanical stress in mice. In: International Journal of Medical Sciences. 2015 ; Vol. 12, No. 9. pp. 689-694.
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abstract = "The purpose of the study was to determine the cell dynamics in periodontal ligament in response to mechanical stress during orthodontic movement. Following Waldo’s method, a square sheet of rubber dam was inserted in between the first and second maxillary molars in 10 ddY mice leaving the stress load for 3 hours. After 3 days and at 1 week, cell count on pressure and tension sides of the periodontal ligament was determined. Furthermore, the type of cell present after mechanical stress was identified using GFP bone marrow transplantation mouse model. Immunohistochemistry was carried out at 0 min (immediately after mechanical stress), 24 hours, 1 week, 2 weeks and 6 months. Temporal changes in the expression of GFP-positive bone marrow derived cells were examined. Moreover, double immunofluorescent staining was performed to determine the type of cell in the periodontal ligament. Cell count on the tension side tremendously increased 3 days after mechanical stress. At 1 week, spindle and round cell count increased compared to the control group. These changes were observed on both tension and pressure sides. Cell count on pressure side at 3 days (22.11+/-13.98) and at 1 week (33.23+/-11.39) was higher compared to the control group (15.26+/-8.29). On the tension side, there was a significantly increased at 3 days (35.46+/-11.85), but decreased at 1 week (29.23+/-13.89) although it is still higher compared to the control group (AD+/-SD: 10.37+/-8.69). Using GFP bone marrow transplantation mouse model, GFP positive cell count increased gradually over time in 6 months. GFP positive cells were also positive to CD31, CD68 and Runx2 suggesting that fibroblasts differentiated into osteoclasts and tissue macrophages. In conclusion, mechanical stress during orthodontic movement promoted the increase in the number of cells in the periodontal ligament on both tension and pressure sides. The increase in the number of cells in the periodontal ligament is believed to be due to the migration and cell division of undifferentiated mesenchymal cells.",
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AU - Iwasaki, Takami

AU - Tomida, Mihoko

AU - Tsujigiwa, Hidetsugu

AU - Nagatsuka, Hitoshi

AU - Kawakami, Toshiyuki

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