Histological comparison of bone induced from autogenously grafted periosteum with bone induced from autogenously grafted bone marrow in the rat calvarial defect model

Takaaki Ueno, Kozo Honda, Azumi Hirata, Toshimasa Kagawa, Miwa Kanou, Nobuaki Shirasu, Masako Sawaki, Eiki Yamachika, Nobuyoshi Mizukawa, Toshio Sugahara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Both periosteum and bone marrow have the potential to induce heterotopic bone when grafted. Whether the process of bone formation is controlled by the recipient environment where the donor graft is placed or by factors from the donor site is not well documented. The purpose of this study was to examine the histology of new bone induced by either autogenously grafted periosteum or autogenously grafted bone marrow using the rat calvarial defect model in Sprague-Dawley rats. Grafts of either bone marrow or periosteum obtained from tibias were placed in calvarial defects with beta-tricalcium phosphate. Ten days after grafting, active cell proliferation was observed in the defects of both types of grafts. After 20 days, cancellous bone formation was observed in the defects with bone marrow grafts, and intramembranous bone formation was observed in the defects with periosteal grafts. After 30 days, bone marrow grafts had developed bone with a bone marrow-like structure, and the periosteal grafts had produced cortical bone structure in the defects. The findings suggest that the type of bone formation is determined by characteristics of the donor site.

Original languageEnglish
Pages (from-to)217-223
Number of pages7
JournalActa Histochemica
Volume110
Issue number3
DOIs
Publication statusPublished - May 22 2008

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Periosteum
Bone Marrow
Transplants
Bone and Bones
Osteogenesis
Controlled Environment
Tibia
Sprague Dawley Rats
Histology
Cell Proliferation

Keywords

  • Bone marrow graft
  • Calvaria
  • Periosteal graft
  • Rat
  • Tricalcium phosphate

ASJC Scopus subject areas

  • Cell Biology

Cite this

Histological comparison of bone induced from autogenously grafted periosteum with bone induced from autogenously grafted bone marrow in the rat calvarial defect model. / Ueno, Takaaki; Honda, Kozo; Hirata, Azumi; Kagawa, Toshimasa; Kanou, Miwa; Shirasu, Nobuaki; Sawaki, Masako; Yamachika, Eiki; Mizukawa, Nobuyoshi; Sugahara, Toshio.

In: Acta Histochemica, Vol. 110, No. 3, 22.05.2008, p. 217-223.

Research output: Contribution to journalArticle

Ueno, Takaaki ; Honda, Kozo ; Hirata, Azumi ; Kagawa, Toshimasa ; Kanou, Miwa ; Shirasu, Nobuaki ; Sawaki, Masako ; Yamachika, Eiki ; Mizukawa, Nobuyoshi ; Sugahara, Toshio. / Histological comparison of bone induced from autogenously grafted periosteum with bone induced from autogenously grafted bone marrow in the rat calvarial defect model. In: Acta Histochemica. 2008 ; Vol. 110, No. 3. pp. 217-223.
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