Histological analysis of microencapsulated dopamine-secreting cells in agarose/poly(styrene sulfonic acid) mixed gel xenotransplanted into the brain

Yasuyuki Miyoshi, Isao Date, Takashi Ohmoto, Hiroo Iwata

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

PC12 cells were encapsulated within agarose/poly(styrene sulfonic acid) (agarose/PSSa) mixed gel and xenotransplanted into the brains of guinea pigs. The immunoprotection capability and biocompatibility of the agarose/PSSa microcapsules were evaluated. Tyrosine hydroxylase-positive PC12 cells within the microcapsules were observed in all guinea pigs at least 5 weeks after transplantation. PC12 cells had a relatively uniform small size and occasionally formed cell clusters; however, cell necrosis was not apparent. The host reaction to agarose/PSSa microcapsules was minimal. The degree of glial fibrillary acidic protein-positive astrocyte density around the microcapsules was similar to that around the injection tracks. Necrosis around the brain/microcapsule interface was not apparent as assessed by Nissl staining. Normal-appearing neurons were observed in close vicinity to the capsule. High-performance liquid chromatography with electrochemical detection showed basal and potassium-evoked release of dopamine from the PC12 cell-enclosed microcapsules in vitro. We conclude that encapsulation using agarose/PSSa mixed gel can not only isolate the enclosed cells from the host immune system but can also allow diffusional exchange of nutrients and neurotransmitters. Encapsulation using agarose/PSSa mixed gel may expand the range of donor tissues for neural transplantation.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalExperimental Neurology
Volume138
Issue number1
DOIs
Publication statusPublished - Mar 1996

Fingerprint

Styrene
Sulfonic Acids
Sepharose
Capsules
Dopamine
Gels
PC12 Cells
Brain
Guinea Pigs
Necrosis
Tissue Transplantation
Glial Fibrillary Acidic Protein
Tyrosine 3-Monooxygenase
Astrocytes
Neurotransmitter Agents
Immune System
Potassium
Transplantation
High Pressure Liquid Chromatography
Staining and Labeling

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Histological analysis of microencapsulated dopamine-secreting cells in agarose/poly(styrene sulfonic acid) mixed gel xenotransplanted into the brain. / Miyoshi, Yasuyuki; Date, Isao; Ohmoto, Takashi; Iwata, Hiroo.

In: Experimental Neurology, Vol. 138, No. 1, 03.1996, p. 169-175.

Research output: Contribution to journalArticle

@article{93f18176c0b84bcd85f30b6d67e8b197,
title = "Histological analysis of microencapsulated dopamine-secreting cells in agarose/poly(styrene sulfonic acid) mixed gel xenotransplanted into the brain",
abstract = "PC12 cells were encapsulated within agarose/poly(styrene sulfonic acid) (agarose/PSSa) mixed gel and xenotransplanted into the brains of guinea pigs. The immunoprotection capability and biocompatibility of the agarose/PSSa microcapsules were evaluated. Tyrosine hydroxylase-positive PC12 cells within the microcapsules were observed in all guinea pigs at least 5 weeks after transplantation. PC12 cells had a relatively uniform small size and occasionally formed cell clusters; however, cell necrosis was not apparent. The host reaction to agarose/PSSa microcapsules was minimal. The degree of glial fibrillary acidic protein-positive astrocyte density around the microcapsules was similar to that around the injection tracks. Necrosis around the brain/microcapsule interface was not apparent as assessed by Nissl staining. Normal-appearing neurons were observed in close vicinity to the capsule. High-performance liquid chromatography with electrochemical detection showed basal and potassium-evoked release of dopamine from the PC12 cell-enclosed microcapsules in vitro. We conclude that encapsulation using agarose/PSSa mixed gel can not only isolate the enclosed cells from the host immune system but can also allow diffusional exchange of nutrients and neurotransmitters. Encapsulation using agarose/PSSa mixed gel may expand the range of donor tissues for neural transplantation.",
author = "Yasuyuki Miyoshi and Isao Date and Takashi Ohmoto and Hiroo Iwata",
year = "1996",
month = "3",
doi = "10.1006/exnr.1996.0055",
language = "English",
volume = "138",
pages = "169--175",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Histological analysis of microencapsulated dopamine-secreting cells in agarose/poly(styrene sulfonic acid) mixed gel xenotransplanted into the brain

AU - Miyoshi, Yasuyuki

AU - Date, Isao

AU - Ohmoto, Takashi

AU - Iwata, Hiroo

PY - 1996/3

Y1 - 1996/3

N2 - PC12 cells were encapsulated within agarose/poly(styrene sulfonic acid) (agarose/PSSa) mixed gel and xenotransplanted into the brains of guinea pigs. The immunoprotection capability and biocompatibility of the agarose/PSSa microcapsules were evaluated. Tyrosine hydroxylase-positive PC12 cells within the microcapsules were observed in all guinea pigs at least 5 weeks after transplantation. PC12 cells had a relatively uniform small size and occasionally formed cell clusters; however, cell necrosis was not apparent. The host reaction to agarose/PSSa microcapsules was minimal. The degree of glial fibrillary acidic protein-positive astrocyte density around the microcapsules was similar to that around the injection tracks. Necrosis around the brain/microcapsule interface was not apparent as assessed by Nissl staining. Normal-appearing neurons were observed in close vicinity to the capsule. High-performance liquid chromatography with electrochemical detection showed basal and potassium-evoked release of dopamine from the PC12 cell-enclosed microcapsules in vitro. We conclude that encapsulation using agarose/PSSa mixed gel can not only isolate the enclosed cells from the host immune system but can also allow diffusional exchange of nutrients and neurotransmitters. Encapsulation using agarose/PSSa mixed gel may expand the range of donor tissues for neural transplantation.

AB - PC12 cells were encapsulated within agarose/poly(styrene sulfonic acid) (agarose/PSSa) mixed gel and xenotransplanted into the brains of guinea pigs. The immunoprotection capability and biocompatibility of the agarose/PSSa microcapsules were evaluated. Tyrosine hydroxylase-positive PC12 cells within the microcapsules were observed in all guinea pigs at least 5 weeks after transplantation. PC12 cells had a relatively uniform small size and occasionally formed cell clusters; however, cell necrosis was not apparent. The host reaction to agarose/PSSa microcapsules was minimal. The degree of glial fibrillary acidic protein-positive astrocyte density around the microcapsules was similar to that around the injection tracks. Necrosis around the brain/microcapsule interface was not apparent as assessed by Nissl staining. Normal-appearing neurons were observed in close vicinity to the capsule. High-performance liquid chromatography with electrochemical detection showed basal and potassium-evoked release of dopamine from the PC12 cell-enclosed microcapsules in vitro. We conclude that encapsulation using agarose/PSSa mixed gel can not only isolate the enclosed cells from the host immune system but can also allow diffusional exchange of nutrients and neurotransmitters. Encapsulation using agarose/PSSa mixed gel may expand the range of donor tissues for neural transplantation.

UR - http://www.scopus.com/inward/record.url?scp=0029871952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029871952&partnerID=8YFLogxK

U2 - 10.1006/exnr.1996.0055

DO - 10.1006/exnr.1996.0055

M3 - Article

VL - 138

SP - 169

EP - 175

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 1

ER -