Histochemical and fine structural study of bone of ipriflavone-treated rats

Hidehiro Ozawa, Hiroaki Nakamura, Kazuharu Irie, Mika Ikegame

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bone labeling, histochemical, and fine structural studies were performed in order to clarify the effects of ipriflavone (IP) on rat bone tissue in vivo and in vitro. Labeling experiments showed a slight increase in bone formation during 3 days' administration. It was also noted that many osteoclasts detached from the bone surface at 1, 2, and 6 hours after administration in vivo. In addition, irregular localization of tartrate-resistant acid phosphatase (TRACPase) activity was observed in osteoclasts. Fine structurally, IP-treated osteoclasts exhibited irregularity in their ruffled borders, as reported in calcitonin administration, and many enlarged rough endoplasmic reticuli and vacuoles were observed. However, osteoclasts at 12 hours after administration, as well as the control, indicated recovery features from the effect of IP. Osteoblast proliferation and differentiation led to increasing alkaline phosphatase activity (ALPase) with time as well as the development of rough endoplasmic reticuli and Golgi apparatus with well-developed fine structure. These findings imply active synthesis of bone matrix. In our in vitro experiment, osteoclasts and osteoblasts displayed histochemical and fine structural characteristics Similar to those observed in our in vivo experiment. Moreover, fewer TRACP-positive mononuclear cells were observed after 24-hour culture with IP than with the control. These results suggest that IP inhibits directly and/or indirectly differentiation and activity of osteoclasts and also promotes differentiation of osteoblast-lineage cells and their bone-forming activity.

Original languageEnglish
JournalCalcified Tissue International
Volume51
Issue number1 Supplement
DOIs
Publication statusPublished - Jan 1992
Externally publishedYes

Fingerprint

Osteoclasts
Bone and Bones
Osteoblasts
Bone Matrix
Calcitonin
Golgi Apparatus
Vacuoles
Osteogenesis
Alkaline Phosphatase
ipriflavone

Keywords

  • Bone resorption
  • Confocal scanning laser microscope
  • Ipriflavone
  • Osteogenesis

ASJC Scopus subject areas

  • Endocrinology

Cite this

Histochemical and fine structural study of bone of ipriflavone-treated rats. / Ozawa, Hidehiro; Nakamura, Hiroaki; Irie, Kazuharu; Ikegame, Mika.

In: Calcified Tissue International, Vol. 51, No. 1 Supplement, 01.1992.

Research output: Contribution to journalArticle

Ozawa, Hidehiro ; Nakamura, Hiroaki ; Irie, Kazuharu ; Ikegame, Mika. / Histochemical and fine structural study of bone of ipriflavone-treated rats. In: Calcified Tissue International. 1992 ; Vol. 51, No. 1 Supplement.
@article{6a0ac94706b14fb4a75f493097a03d81,
title = "Histochemical and fine structural study of bone of ipriflavone-treated rats",
abstract = "Bone labeling, histochemical, and fine structural studies were performed in order to clarify the effects of ipriflavone (IP) on rat bone tissue in vivo and in vitro. Labeling experiments showed a slight increase in bone formation during 3 days' administration. It was also noted that many osteoclasts detached from the bone surface at 1, 2, and 6 hours after administration in vivo. In addition, irregular localization of tartrate-resistant acid phosphatase (TRACPase) activity was observed in osteoclasts. Fine structurally, IP-treated osteoclasts exhibited irregularity in their ruffled borders, as reported in calcitonin administration, and many enlarged rough endoplasmic reticuli and vacuoles were observed. However, osteoclasts at 12 hours after administration, as well as the control, indicated recovery features from the effect of IP. Osteoblast proliferation and differentiation led to increasing alkaline phosphatase activity (ALPase) with time as well as the development of rough endoplasmic reticuli and Golgi apparatus with well-developed fine structure. These findings imply active synthesis of bone matrix. In our in vitro experiment, osteoclasts and osteoblasts displayed histochemical and fine structural characteristics Similar to those observed in our in vivo experiment. Moreover, fewer TRACP-positive mononuclear cells were observed after 24-hour culture with IP than with the control. These results suggest that IP inhibits directly and/or indirectly differentiation and activity of osteoclasts and also promotes differentiation of osteoblast-lineage cells and their bone-forming activity.",
keywords = "Bone resorption, Confocal scanning laser microscope, Ipriflavone, Osteogenesis",
author = "Hidehiro Ozawa and Hiroaki Nakamura and Kazuharu Irie and Mika Ikegame",
year = "1992",
month = "1",
doi = "10.1007/BF02180245",
language = "English",
volume = "51",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York",
number = "1 Supplement",

}

TY - JOUR

T1 - Histochemical and fine structural study of bone of ipriflavone-treated rats

AU - Ozawa, Hidehiro

AU - Nakamura, Hiroaki

AU - Irie, Kazuharu

AU - Ikegame, Mika

PY - 1992/1

Y1 - 1992/1

N2 - Bone labeling, histochemical, and fine structural studies were performed in order to clarify the effects of ipriflavone (IP) on rat bone tissue in vivo and in vitro. Labeling experiments showed a slight increase in bone formation during 3 days' administration. It was also noted that many osteoclasts detached from the bone surface at 1, 2, and 6 hours after administration in vivo. In addition, irregular localization of tartrate-resistant acid phosphatase (TRACPase) activity was observed in osteoclasts. Fine structurally, IP-treated osteoclasts exhibited irregularity in their ruffled borders, as reported in calcitonin administration, and many enlarged rough endoplasmic reticuli and vacuoles were observed. However, osteoclasts at 12 hours after administration, as well as the control, indicated recovery features from the effect of IP. Osteoblast proliferation and differentiation led to increasing alkaline phosphatase activity (ALPase) with time as well as the development of rough endoplasmic reticuli and Golgi apparatus with well-developed fine structure. These findings imply active synthesis of bone matrix. In our in vitro experiment, osteoclasts and osteoblasts displayed histochemical and fine structural characteristics Similar to those observed in our in vivo experiment. Moreover, fewer TRACP-positive mononuclear cells were observed after 24-hour culture with IP than with the control. These results suggest that IP inhibits directly and/or indirectly differentiation and activity of osteoclasts and also promotes differentiation of osteoblast-lineage cells and their bone-forming activity.

AB - Bone labeling, histochemical, and fine structural studies were performed in order to clarify the effects of ipriflavone (IP) on rat bone tissue in vivo and in vitro. Labeling experiments showed a slight increase in bone formation during 3 days' administration. It was also noted that many osteoclasts detached from the bone surface at 1, 2, and 6 hours after administration in vivo. In addition, irregular localization of tartrate-resistant acid phosphatase (TRACPase) activity was observed in osteoclasts. Fine structurally, IP-treated osteoclasts exhibited irregularity in their ruffled borders, as reported in calcitonin administration, and many enlarged rough endoplasmic reticuli and vacuoles were observed. However, osteoclasts at 12 hours after administration, as well as the control, indicated recovery features from the effect of IP. Osteoblast proliferation and differentiation led to increasing alkaline phosphatase activity (ALPase) with time as well as the development of rough endoplasmic reticuli and Golgi apparatus with well-developed fine structure. These findings imply active synthesis of bone matrix. In our in vitro experiment, osteoclasts and osteoblasts displayed histochemical and fine structural characteristics Similar to those observed in our in vivo experiment. Moreover, fewer TRACP-positive mononuclear cells were observed after 24-hour culture with IP than with the control. These results suggest that IP inhibits directly and/or indirectly differentiation and activity of osteoclasts and also promotes differentiation of osteoblast-lineage cells and their bone-forming activity.

KW - Bone resorption

KW - Confocal scanning laser microscope

KW - Ipriflavone

KW - Osteogenesis

UR - http://www.scopus.com/inward/record.url?scp=0027076890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027076890&partnerID=8YFLogxK

U2 - 10.1007/BF02180245

DO - 10.1007/BF02180245

M3 - Article

VL - 51

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 1 Supplement

ER -