Histidine decarboxylase expression in mouse mast cell line P815 is induced by mouse peritoneal cavity incubation

Hiroshi Ohtsu, Atsuo Kuramasu, Satsuki Suzuki, Kazuhiko Igarashi, Yuzuru Ohuchi, Maki Sato, Satoshi Tanaka, Satoshi Nakagawa, Kunio Shirato, Masayuki Yamamoto, Atsushi Ichikawa, Takehiko Watanabe

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Phenotype of P815 mouse mast cells changes markedly during culture in the peritoneal cavity of syngenic BDF1 mice. The cells, cultured for 1 week in the peritoneal cavity of syngenic BDF1 mice, proliferate and express high levels of L-histidine decarboxylase (HDC) and mouse mast cell protease (MMCP)-6 mRNAs, indicating the ability of P815 cells to differentiate toward mature connective tissue mast cells. Peritoneal fluid aspirated from P815- inoculated BDF1 mouse and added to cultured P815 cells in vitro was also found to induce HDC mRNA expression, suggesting that at least some of the humoral factors in the peritoneal fluid induce HDC mRNA transcription. Among the erythroid transcription factors, P815 cells expressed GATA-2 but not GATA-1 mRNA before and after the intraperitoneal incubation. In contrast, the expression of NF-E2 subunit p45 disappeared, while expression of subunit mafK was markedly reduced after incubation. Cotransfection assays using HDC- luciferase reporter and p45 and/or mark expression constructs showed that NF- E2 affects the transactivation of HDC gene. These results suggest that NF-E2 is also an important transcription factor in mast cell differentiation.

Original languageEnglish
Pages (from-to)28439-28444
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number45
DOIs
Publication statusPublished - 1996
Externally publishedYes

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Histidine Decarboxylase
Peritoneal Cavity
Histidine
Mast Cells
Cells
Cell Line
Messenger RNA
Ascitic Fluid
Cultured Cells
Transcription Factors
Connective Tissue Cells
Fluids
Transcription
Luciferases
Transcriptional Activation
Cell Differentiation
Assays
Genes
Tissue
Phenotype

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ohtsu, H., Kuramasu, A., Suzuki, S., Igarashi, K., Ohuchi, Y., Sato, M., ... Watanabe, T. (1996). Histidine decarboxylase expression in mouse mast cell line P815 is induced by mouse peritoneal cavity incubation. Journal of Biological Chemistry, 271(45), 28439-28444. https://doi.org/10.1074/jbc.271.45.28439

Histidine decarboxylase expression in mouse mast cell line P815 is induced by mouse peritoneal cavity incubation. / Ohtsu, Hiroshi; Kuramasu, Atsuo; Suzuki, Satsuki; Igarashi, Kazuhiko; Ohuchi, Yuzuru; Sato, Maki; Tanaka, Satoshi; Nakagawa, Satoshi; Shirato, Kunio; Yamamoto, Masayuki; Ichikawa, Atsushi; Watanabe, Takehiko.

In: Journal of Biological Chemistry, Vol. 271, No. 45, 1996, p. 28439-28444.

Research output: Contribution to journalArticle

Ohtsu, H, Kuramasu, A, Suzuki, S, Igarashi, K, Ohuchi, Y, Sato, M, Tanaka, S, Nakagawa, S, Shirato, K, Yamamoto, M, Ichikawa, A & Watanabe, T 1996, 'Histidine decarboxylase expression in mouse mast cell line P815 is induced by mouse peritoneal cavity incubation', Journal of Biological Chemistry, vol. 271, no. 45, pp. 28439-28444. https://doi.org/10.1074/jbc.271.45.28439
Ohtsu, Hiroshi ; Kuramasu, Atsuo ; Suzuki, Satsuki ; Igarashi, Kazuhiko ; Ohuchi, Yuzuru ; Sato, Maki ; Tanaka, Satoshi ; Nakagawa, Satoshi ; Shirato, Kunio ; Yamamoto, Masayuki ; Ichikawa, Atsushi ; Watanabe, Takehiko. / Histidine decarboxylase expression in mouse mast cell line P815 is induced by mouse peritoneal cavity incubation. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 45. pp. 28439-28444.
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AU - Tanaka, Satoshi

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