Histatin as a synergistic stimulator with epidermal growth factor of rabbit chondrocyte proliferation

Yukitaka Murakami, Hideki Nagata, Satoshi Shizukuishi, Kazuhisa Nakashima, Tokutaro Okawa, Masaharu Takigawa, Akira Tsunemitsu

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Histatin 5 dose-dependently increased DNA synthesis in rabbit costal chondrocytes in culture. The level of DNA synthesis was stimulated by histatin 5 to about 4 times that of the control. The combination of histatin 5 and epidermal growth factor (EGF) increased the DNA synthesis to about 40 times that of the control while EGF alone stimulated it 15-fold, indicating synergistic stimulation by both factors. Enzyme-linked immunosorbent assay using anti-histatin 5 demonstrated that human serum contained histatin-like substances. These findings suggest that histatins play an important role in chondrocyte proliferation, presumably as a physiological modulator of the action of EGF.

Original languageEnglish
Pages (from-to)274-280
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume198
Issue number1
DOIs
Publication statusPublished - Jan 15 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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