Histamine synthesis is required for granule maturation in murine mast cells

Shunsuke Nakazawa, Mariko Sakanaka, Kazuyuki Furuta, Mayuko Natsuhara, Hirotsugu Takano, Soken Tsuchiya, Yasushi Okuno, Hiroshi Ohtsu, Masahiro Nishibori, Robin L. Thurmond, Noriyasu Hirasawa, Kazuhisa Nakayama, Atsushi Ichikawa, Yukihiko Sugimoto, Satoshi Tanaka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mast cells are the major sources of histamine, which is released in response to immunological stimulations. The synthesis of histamine is catalyzed by histidine decarboxylase (HDC). Previous studies have shown that Hdc-/- mast cells exhibit aberrant granule morphology with severely decreased granule content. Here, we investigated whether the histamine synthesized in mast cells regulates the granule maturation of murine mast cells. Several genes, including those encoding granule proteases and enzymes involved in heparin biosynthesis, were downregulated in Hdc-/- peritoneal mast cells. Impaired granule maturation was also found in Hdc-/- BM-derived cultured mast cells when they were cocultured with fibroblasts in the presence of c-kit ligand. Exogenous application of histamine and several H4 receptor agonists restored the granule maturation of Hdc-/- cultured mast cells. However, the maturation of granules was largely normal in Hrh4-/- peritoneal mast cells. Depletion of cellular histamine with tetrabenazine, an inhibitor of vesicular monoamine transporter-2, did not affect granule maturation. In vivo experiments with mast cell deficient KitW/KitW-v mice indicated that the expression of the Hdc gene in mast cells is required for granule maturation. These results suggest that histamine promotes granule maturation in mast cells and acts as an proinflammatory mediator.

Original languageEnglish
Pages (from-to)204-214
Number of pages11
JournalEuropean Journal of Immunology
Volume44
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Mast Cells
Histamine
Cultured Cells
Tetrabenazine
Vesicular Monoamine Transport Proteins
Histidine Decarboxylase
Stem Cell Factor
Heparin
Immunization
Peptide Hydrolases
Down-Regulation
Fibroblasts
Gene Expression

Keywords

  • Cell differentiation
  • Histamine
  • Inflammation
  • Mast cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Medicine(all)

Cite this

Nakazawa, S., Sakanaka, M., Furuta, K., Natsuhara, M., Takano, H., Tsuchiya, S., ... Tanaka, S. (2014). Histamine synthesis is required for granule maturation in murine mast cells. European Journal of Immunology, 44(1), 204-214. https://doi.org/10.1002/eji.201343838

Histamine synthesis is required for granule maturation in murine mast cells. / Nakazawa, Shunsuke; Sakanaka, Mariko; Furuta, Kazuyuki; Natsuhara, Mayuko; Takano, Hirotsugu; Tsuchiya, Soken; Okuno, Yasushi; Ohtsu, Hiroshi; Nishibori, Masahiro; Thurmond, Robin L.; Hirasawa, Noriyasu; Nakayama, Kazuhisa; Ichikawa, Atsushi; Sugimoto, Yukihiko; Tanaka, Satoshi.

In: European Journal of Immunology, Vol. 44, No. 1, 2014, p. 204-214.

Research output: Contribution to journalArticle

Nakazawa, S, Sakanaka, M, Furuta, K, Natsuhara, M, Takano, H, Tsuchiya, S, Okuno, Y, Ohtsu, H, Nishibori, M, Thurmond, RL, Hirasawa, N, Nakayama, K, Ichikawa, A, Sugimoto, Y & Tanaka, S 2014, 'Histamine synthesis is required for granule maturation in murine mast cells', European Journal of Immunology, vol. 44, no. 1, pp. 204-214. https://doi.org/10.1002/eji.201343838
Nakazawa, Shunsuke ; Sakanaka, Mariko ; Furuta, Kazuyuki ; Natsuhara, Mayuko ; Takano, Hirotsugu ; Tsuchiya, Soken ; Okuno, Yasushi ; Ohtsu, Hiroshi ; Nishibori, Masahiro ; Thurmond, Robin L. ; Hirasawa, Noriyasu ; Nakayama, Kazuhisa ; Ichikawa, Atsushi ; Sugimoto, Yukihiko ; Tanaka, Satoshi. / Histamine synthesis is required for granule maturation in murine mast cells. In: European Journal of Immunology. 2014 ; Vol. 44, No. 1. pp. 204-214.
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