Histamine release from rat mast cells induced by human α-defensin-1 present in jaw cyst fluid

Nobuyoshi Mizukawa, Koichi Sawaki, Masayuki Kamio, Joji Fukunaga, Takaaki Ueno, Shin Takagi, Toshio Sugahara

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the release of histamine from rat mast cells elicited by human α-defensin-1 contained in jaw cyst fluid. Patients and Methods: Human α-defensin-1 was purified by reversed-phase high-performance liquid chromatography from human jaw cyst fluid, and the mast cells were collected from the peritoneal cavities of rats. Results: Histamine release was induced in isolated rat mast cells by both extracted and synthetic human α-defensin-1. Changes in vascular permeability induced by human α-defensin-1 were also estimated by the passive skin test in rats. Extracted and synthetic human α-defensin-1 induced histamine release from isolated rat mast cells in a dose-dependent manner over a concentration range of 1 to 15 μg/mL; the histamine release induced by the extracted and synthetic human α-defensin-1 (15 μg/mL) was 39.4 ± 2.3 and 37.6 ± 1.8%, respectively. When human α-defensin-1 was injected into rat skin intradermally, the vascular permeability increased. This response occurred with as little as 0.1 μg of human α-defensin-1, and a strong response was observed with 0.5 and 1.0 μg. These responses were completely abolished upon injection with 1μg of the antihistamine drug diphenhydramine hydrochloride. Conclusion: Human α-defensin-1 in human jaw cyst fluid can act as an inducer of histamine release from mast cells both in vivo and in vitro.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalAsian Journal of Oral and Maxillofacial Surgery
Volume14
Issue number1
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Jaw Cysts
Cyst Fluid
Defensins
Histamine Release
Mast Cells
Capillary Permeability
Diphenhydramine
Histamine Antagonists
Peritoneal Cavity
Reverse-Phase Chromatography
Skin Tests

Keywords

  • Cyst fluid
  • Defensins
  • Histamine release
  • Jaw cysts
  • Mast cells

ASJC Scopus subject areas

  • Surgery

Cite this

Mizukawa, N., Sawaki, K., Kamio, M., Fukunaga, J., Ueno, T., Takagi, S., & Sugahara, T. (2002). Histamine release from rat mast cells induced by human α-defensin-1 present in jaw cyst fluid. Asian Journal of Oral and Maxillofacial Surgery, 14(1), 35-39.

Histamine release from rat mast cells induced by human α-defensin-1 present in jaw cyst fluid. / Mizukawa, Nobuyoshi; Sawaki, Koichi; Kamio, Masayuki; Fukunaga, Joji; Ueno, Takaaki; Takagi, Shin; Sugahara, Toshio.

In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 14, No. 1, 2002, p. 35-39.

Research output: Contribution to journalArticle

Mizukawa, N, Sawaki, K, Kamio, M, Fukunaga, J, Ueno, T, Takagi, S & Sugahara, T 2002, 'Histamine release from rat mast cells induced by human α-defensin-1 present in jaw cyst fluid', Asian Journal of Oral and Maxillofacial Surgery, vol. 14, no. 1, pp. 35-39.
Mizukawa, Nobuyoshi ; Sawaki, Koichi ; Kamio, Masayuki ; Fukunaga, Joji ; Ueno, Takaaki ; Takagi, Shin ; Sugahara, Toshio. / Histamine release from rat mast cells induced by human α-defensin-1 present in jaw cyst fluid. In: Asian Journal of Oral and Maxillofacial Surgery. 2002 ; Vol. 14, No. 1. pp. 35-39.
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