TY - JOUR
T1 - Histamine release from rat mast cells by Vibrio vulnificus protease
AU - Miyoshi, Shin ichi
AU - Sugiyama, Katsumi
AU - Furuta, Hiroshi
AU - Miyoshi, Noriko
AU - Shinoda, Sumio
PY - 1986/5
Y1 - 1986/5
N2 - Vibrio vulnificus protease (VVP) stimulated histamine release from isolated mast cells in a dose- and temperature-dependent manner within a range of 0.2-4.0 μg/0.5 ml. Histamine release was accompanied by degranulation, and no leakage of lactate dehydrogenase from cells was observed, indicating that the histamine release was not due to cytolysis but to exocytosis. This release, completed within 30 s at 37°C, suggested that the mechanism of action of VVP on mast cells is different from that of other proteases, such as trypsin or α-chymotrypsin, which release histamine from the cells slowly.
AB - Vibrio vulnificus protease (VVP) stimulated histamine release from isolated mast cells in a dose- and temperature-dependent manner within a range of 0.2-4.0 μg/0.5 ml. Histamine release was accompanied by degranulation, and no leakage of lactate dehydrogenase from cells was observed, indicating that the histamine release was not due to cytolysis but to exocytosis. This release, completed within 30 s at 37°C, suggested that the mechanism of action of VVP on mast cells is different from that of other proteases, such as trypsin or α-chymotrypsin, which release histamine from the cells slowly.
KW - Edema
KW - hemoconcentration
KW - vascular permeability
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U2 - 10.1111/j.1574-6968.1986.tb01425.x
DO - 10.1111/j.1574-6968.1986.tb01425.x
M3 - Article
AN - SCOPUS:0022623755
SN - 0378-1097
VL - 34
SP - 301
EP - 304
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 3
ER -