Histamine release from rat mast cells by Vibrio vulnificus protease

Shin ichi Miyoshi, Katsumi Sugiyama, Hiroshi Furuta, Noriko Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Vibrio vulnificus protease (VVP) stimulated histamine release from isolated mast cells in a dose- and temperature-dependent manner within a range of 0.2-4.0 μg/0.5 ml. Histamine release was accompanied by degranulation, and no leakage of lactate dehydrogenase from cells was observed, indicating that the histamine release was not due to cytolysis but to exocytosis. This release, completed within 30 s at 37°C, suggested that the mechanism of action of VVP on mast cells is different from that of other proteases, such as trypsin or α-chymotrypsin, which release histamine from the cells slowly.

Original languageEnglish
Pages (from-to)301-304
Number of pages4
JournalFEMS Microbiology Letters
Issue number3
Publication statusPublished - May 1986


  • Edema
  • hemoconcentration
  • vascular permeability

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


Dive into the research topics of 'Histamine release from rat mast cells by Vibrio vulnificus protease'. Together they form a unique fingerprint.

Cite this