Histamine release from rat mast cells by Vibrio vulnificus protease

Shin-ichi Miyoshi, Katsumi Sugiyama, Hiroshi Furuta, Noriko Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Vibrio vulnificus protease (VVP) stimulated histamine release from isolated mast cells in a dose- and temperature-dependent manner within a range of 0.2-4.0 μg/0.5 ml. Histamine release was accompanied by degranulation, and no leakage of lactate dehydrogenase from cells was observed, indicating that the histamine release was not due to cytolysis but to exocytosis. This release, completed within 30 s at 37°C, suggested that the mechanism of action of VVP on mast cells is different from that of other proteases, such as trypsin or α-chymotrypsin, which release histamine from the cells slowly.

Original languageEnglish
Pages (from-to)301-304
Number of pages4
JournalFEMS Microbiology Letters
Volume34
Issue number3
Publication statusPublished - 1986

Fingerprint

Vibrio vulnificus
Histamine Release
Mast Cells
Peptide Hydrolases
Exocytosis
L-Lactate Dehydrogenase
Temperature

Keywords

  • Edema
  • hemoconcentration
  • vascular permeability

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Miyoshi, S., Sugiyama, K., Furuta, H., Miyoshi, N., & Shinoda, S. (1986). Histamine release from rat mast cells by Vibrio vulnificus protease. FEMS Microbiology Letters, 34(3), 301-304.

Histamine release from rat mast cells by Vibrio vulnificus protease. / Miyoshi, Shin-ichi; Sugiyama, Katsumi; Furuta, Hiroshi; Miyoshi, Noriko; Shinoda, Sumio.

In: FEMS Microbiology Letters, Vol. 34, No. 3, 1986, p. 301-304.

Research output: Contribution to journalArticle

Miyoshi, S, Sugiyama, K, Furuta, H, Miyoshi, N & Shinoda, S 1986, 'Histamine release from rat mast cells by Vibrio vulnificus protease', FEMS Microbiology Letters, vol. 34, no. 3, pp. 301-304.
Miyoshi S, Sugiyama K, Furuta H, Miyoshi N, Shinoda S. Histamine release from rat mast cells by Vibrio vulnificus protease. FEMS Microbiology Letters. 1986;34(3):301-304.
Miyoshi, Shin-ichi ; Sugiyama, Katsumi ; Furuta, Hiroshi ; Miyoshi, Noriko ; Shinoda, Sumio. / Histamine release from rat mast cells by Vibrio vulnificus protease. In: FEMS Microbiology Letters. 1986 ; Vol. 34, No. 3. pp. 301-304.
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