Histamine inhibits lipopolysaccharide-induced interleukin (IL)-18 production in human monocytes

Hideo Kohka Takahashi; Hiromi Iwagaki; Shuji Mori; Tadashi Yoshino; Noriaki Tanaka; Masahiro Nishibori

doi:10.1016/j.clim.2004.03.006

Histamine inhibits lipopolysaccharide-induced interleukin (IL)-18 production in human monocytes

Hideo Kohka Takahashi, Hiromi Iwagaki, Shuji Mori, Tadashi Yoshino, Noriaki Tanaka, Masahiro Nishibori

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS) is an inducer of interleukin (IL)-18, which in turn plays important roles in immune responses. Previously, we reported that tumor necrosis factor (TNF)-α production could be detected in human peripheral blood mononuclear cells (PBMCs) treated with relatively low concentration of LPS (1 ng/ml), but that same concentration of LPS could not induce IL-18 production. In the present study, we found that LPS at relatively high concentrations (10-1000 ng/ml) induced IL-18 production in a concentration-dependent manner both in monocytes isolated from PBMC, and that histamine (10^-7 to 10^-4 M) inhibited IL-18 production induced by LPS. The studies using receptor subtype-selective agonists and antagonists suggested that the effect of histamine was mediated by H2 receptor but not by H1, H3 and H4 receptors. Therefore, the stimulation of H2 receptor might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.

Original language	English
Pages (from-to)	30-34
Number of pages	5
Journal	Clinical Immunology
Volume	112
Issue number	1
DOIs	https://doi.org/10.1016/j.clim.2004.03.006
Publication status	Published - Jul 2004

Keywords

H2 receptor
Histamine
Human
IL-18, LPS
Monocyte
PBMC
cAMP

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.clim.2004.03.006

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title = "Histamine inhibits lipopolysaccharide-induced interleukin (IL)-18 production in human monocytes",

abstract = "Lipopolysaccharide (LPS) is an inducer of interleukin (IL)-18, which in turn plays important roles in immune responses. Previously, we reported that tumor necrosis factor (TNF)-α production could be detected in human peripheral blood mononuclear cells (PBMCs) treated with relatively low concentration of LPS (1 ng/ml), but that same concentration of LPS could not induce IL-18 production. In the present study, we found that LPS at relatively high concentrations (10-1000 ng/ml) induced IL-18 production in a concentration-dependent manner both in monocytes isolated from PBMC, and that histamine (10-7 to 10-4 M) inhibited IL-18 production induced by LPS. The studies using receptor subtype-selective agonists and antagonists suggested that the effect of histamine was mediated by H2 receptor but not by H1, H3 and H4 receptors. Therefore, the stimulation of H2 receptor might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.",

keywords = "H2 receptor, Histamine, Human, IL-18, LPS, Monocyte, PBMC, cAMP",

author = "Takahashi, {Hideo Kohka} and Hiromi Iwagaki and Shuji Mori and Tadashi Yoshino and Noriaki Tanaka and Masahiro Nishibori",

note = "Funding Information: The authors thank Drs. Duncan WAM and Durant D-J (The Research Institute, Smith Kline and French Laboratories) for generously donating 2-(2-pyridyl) ethylamine, dimaprit and 4-methylhistamine. This study was supported in part by a grant for Promotion of Research from Okayama University (No.21 to M.N.), a grant from Okayama Medical Foundation (to H.K.T.), and grants from Grant-in-Aid for Scientific Research (C) (15590467 to H.K.T. and 15590228 to M.N.).",

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AU - Takahashi, Hideo Kohka

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AU - Mori, Shuji

AU - Yoshino, Tadashi

AU - Tanaka, Noriaki

AU - Nishibori, Masahiro

N1 - Funding Information: The authors thank Drs. Duncan WAM and Durant D-J (The Research Institute, Smith Kline and French Laboratories) for generously donating 2-(2-pyridyl) ethylamine, dimaprit and 4-methylhistamine. This study was supported in part by a grant for Promotion of Research from Okayama University (No.21 to M.N.), a grant from Okayama Medical Foundation (to H.K.T.), and grants from Grant-in-Aid for Scientific Research (C) (15590467 to H.K.T. and 15590228 to M.N.).

PY - 2004/7

Y1 - 2004/7

N2 - Lipopolysaccharide (LPS) is an inducer of interleukin (IL)-18, which in turn plays important roles in immune responses. Previously, we reported that tumor necrosis factor (TNF)-α production could be detected in human peripheral blood mononuclear cells (PBMCs) treated with relatively low concentration of LPS (1 ng/ml), but that same concentration of LPS could not induce IL-18 production. In the present study, we found that LPS at relatively high concentrations (10-1000 ng/ml) induced IL-18 production in a concentration-dependent manner both in monocytes isolated from PBMC, and that histamine (10-7 to 10-4 M) inhibited IL-18 production induced by LPS. The studies using receptor subtype-selective agonists and antagonists suggested that the effect of histamine was mediated by H2 receptor but not by H1, H3 and H4 receptors. Therefore, the stimulation of H2 receptor might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.

AB - Lipopolysaccharide (LPS) is an inducer of interleukin (IL)-18, which in turn plays important roles in immune responses. Previously, we reported that tumor necrosis factor (TNF)-α production could be detected in human peripheral blood mononuclear cells (PBMCs) treated with relatively low concentration of LPS (1 ng/ml), but that same concentration of LPS could not induce IL-18 production. In the present study, we found that LPS at relatively high concentrations (10-1000 ng/ml) induced IL-18 production in a concentration-dependent manner both in monocytes isolated from PBMC, and that histamine (10-7 to 10-4 M) inhibited IL-18 production induced by LPS. The studies using receptor subtype-selective agonists and antagonists suggested that the effect of histamine was mediated by H2 receptor but not by H1, H3 and H4 receptors. Therefore, the stimulation of H2 receptor might be beneficial in the treatment of sepsis through inhibiting LPS-elicited IL-18.

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KW - PBMC

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Histamine inhibits lipopolysaccharide-induced interleukin (IL)-18 production in human monocytes

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