Highly purified hydroxylamine oxidoreductase derived from Nitrosomonas europaea: Some physicochemical and enzymatic properties

Tateo Yamanaka, Minoru Shinra, Koko Takahashi, Mineo Shibasaka

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Hydroxylamine oxidoreductase [EC 1.7.3.4] of Nitrosomonas europaea was purified to an electrophoretically homogeneous state and some of its properties were studied.The molecular weight of the enzyme as determined by gel filtration on Sephadex G-150 and by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate is 175,000-180,000, while the minimum molecular weight per heme determined from the dry weight and heme content is 17,500. The enzyme is a C-type cytochrome; its reduced form shows absorption peaks at 418 (γ peak), 521 (β peak), 553 (α peak), and 460 nm (due to an unidentified chromophore). Although the α peak at 553 nm has a shoulder at 559 nm, the enzyme does not possess protoheme or a cytochrome b subunit. It seems likely that the enzyme molecule possesses heme c molecules in different states.The enzyme reacts rapidly with various eukaryotic cytochromes c, but does not react with "bacterial-type" cytochromes c. Although the enzyme does not react with cytochrome c-552 (N. europaea), another C-type cytochrome of the organism, cytochrome c-554 (N. europaea) acts as an electron acceptor for the enzyme.

Original languageEnglish
Pages (from-to)1101-1108
Number of pages8
JournalJournal of Biochemistry
Volume86
Issue number4
Publication statusPublished - Oct 1979
Externally publishedYes

Fingerprint

hydroxylamine dehydrogenase
Nitrosomonas europaea
Enzymes
Proteins
Heme
Cytochromes c
Cytochromes
Gels
Molecular Weight
Molecular weight
Molecules
Cytochrome c Group
Cytochromes b
Sodium dodecyl sulfate
Chromophores
Oxidoreductases
Polyacrylates
Electrophoresis
Sodium Dodecyl Sulfate
Sodium

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Highly purified hydroxylamine oxidoreductase derived from Nitrosomonas europaea : Some physicochemical and enzymatic properties. / Yamanaka, Tateo; Shinra, Minoru; Takahashi, Koko; Shibasaka, Mineo.

In: Journal of Biochemistry, Vol. 86, No. 4, 10.1979, p. 1101-1108.

Research output: Contribution to journalArticle

Yamanaka, Tateo ; Shinra, Minoru ; Takahashi, Koko ; Shibasaka, Mineo. / Highly purified hydroxylamine oxidoreductase derived from Nitrosomonas europaea : Some physicochemical and enzymatic properties. In: Journal of Biochemistry. 1979 ; Vol. 86, No. 4. pp. 1101-1108.
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