Highly expressed genes in a rough-colony-forming phenotype of Aggregatibacter actinomycetemcomitans: Implication of a mip-like gene for the invasion of host tissue

Takemasa Maeda, Hiroshi Maeda, Kokoro Yamabe, Junji Mineshiba, Ichiro Tanimoto, Tadashi Yamamoto, Koji Naruishi, Susumu Kokeguchi, Shogo Takashiba

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aggregatibacter actinomycetemcomitans, a potent pathogen of periodontitis, typically grows as a rough and adherent colony on primary isolated cultures. The colony transforms into a smooth phenotype during repeated subculture. In this study, we aimed to identify highly expressed genes in the rough-colony-forming phenotype for isolation of host-induced genes. Using a cDNA-subtractive hybridization technique, three genes, homologous to a macrophage infectivity potentiator gene (mip), peroxiredoxin gene (prx) and outer membrane protein gene (ompA), were identified. The expression levels of these genes in the rough-colony-forming phenotype were 4-10-fold higher as compared with the smooth-colony-forming phenotype. Attention was focused on the mip-like gene, and a recombinant protein and a deficient mutant were constructed. The recombinant protein reacted with sera from patients with periodontitis, suggesting the production of the Mip-like protein in periodontal lesions. Viable quantitative invasion assay demonstrated that the viable cell counts of the wild-type strain that invaded HeLa cells were more than fourfold as compared with the mip-deficient mutant. The expression of the mip-like gene, prx-like gene and ompA-like gene may be enhanced in the host, and the mip-like gene may play an important role in the infection of A. actinomycetemcomitans, especially in its invasion of the epithelium.

Original languageEnglish
Pages (from-to)226-236
Number of pages11
JournalFEMS Immunology and Medical Microbiology
Volume58
Issue number2
DOIs
Publication statusPublished - 2010

Fingerprint

Aggregatibacter actinomycetemcomitans
Macrophages
Phenotype
Genes
Peroxiredoxins
Periodontitis
Recombinant Proteins

Keywords

  • Aggregatibacter actinomycetemcomitans
  • Host-induced gene
  • Invasion
  • Macrophage infectivity potentiator (mip)
  • Rough-colony-forming phenotype

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology
  • Medicine(all)

Cite this

Highly expressed genes in a rough-colony-forming phenotype of Aggregatibacter actinomycetemcomitans : Implication of a mip-like gene for the invasion of host tissue. / Maeda, Takemasa; Maeda, Hiroshi; Yamabe, Kokoro; Mineshiba, Junji; Tanimoto, Ichiro; Yamamoto, Tadashi; Naruishi, Koji; Kokeguchi, Susumu; Takashiba, Shogo.

In: FEMS Immunology and Medical Microbiology, Vol. 58, No. 2, 2010, p. 226-236.

Research output: Contribution to journalArticle

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